タイ寄生虫感染関連胆道がんの症例・対照研究による遺伝子多型の解析

泰国寄生虫感染胆道癌病例对照研究基因多态性分析

• 批准号: 15026204
• 负责人: 三輪 正直
• 金额: $ 2.94万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15026204/
• 关键词: 胆道がん; 肝臓がん; 肝内胆管がん; 肝吸虫感染; GSTM1; GSTT1; ニトロサミン; XRCC1

胆道がん(肝内胆管がん、胆嚢がん、肝外胆管がん)は、早期発見が困難であり予後は極めて不良である。この中で肝内胆管がんは、我国においても比較的少ないが稀ではなく、また、米国においてもここ10年間で増えているとの報告もあり、原因究明と予防へ向けての研究が重要である。タイ王国東北部の肝臓がんの年齢調整罹患率は世界一高率で、その肝臓がんの80%が肝内胆管がんである。本研究は遺伝的多型の差が発がん感受性の個体差を規定しているとの作業仮説を立て、症例・対照研究を行なった。肝吸虫抗体価上昇例では正常例に比べて、胆管細胞がん罹患のリスクが著しく上昇しており、このリスクは、薬物代謝酵素のGSTM1多型による修飾を受けていた。また、飲酒が胆管細胞がん罹患のリスクを有意に上昇させ、GSTM1及びGSTT1多型は、この発がんリスクに影響を及ぼしていることが強く疑われた。この結果をまとめて、現在論文投稿中である。DNA修復遺伝子多型XRCC1の多型に関しての解析では、コドン339の変異が比較的若年者において肝内胆管がんのリスクを高めている可能性が示唆された。一方、肝吸虫抗体価上昇や、飲酒、発酵魚製品摂取との間の交互作用(修飾)は認められなかった。さらに修復関連酵素としてのhOGG1やその他の酵素多型と発がんリスクとの解析を進めている。

Bile duct disease (intrahepatic bile duct disease, bile duct disease, extrahepatic bile duct disease), early diagnosis of poor bile duct, bile duct, bile duct and extrahepatic bile duct. The number of patients with intrahepatic bile duct disease in China, the number of patients in our country, the number of patients in the United States, the United States, and the United States have not been reported for 10 years, and the reasons are clear. In the northern part of the kingdom, the overall incidence rate is one of the highest in the world, 80% in the liver and 80% in the intrahepatic bile duct. In this study, the multi-type differential sensitivity scale stipulated that the operation procedure was established, and the case study was conducted according to the case study. The antibody against clonorchiasis was detected in the liver clonorchiasis antibody, the normal cases were compared, the bile duct cells were infected, and the antibodies were detected in the liver fluke antibody, liver fluke antibody, The bile duct cells of the patients with alcoholic beverages are suffering from the disease. They are interested in developing the GSTM1 and GSTT1 genotypes. The results show that you have received a lot of attention and are now in the process of submitting articles. DNA revises XRCC1 polymorphisms, polymorphisms, phenotypes, paraphernalia, age, intrahepatic bile ducts, liver, liver, bile duct, liver, bile duct, bile duct, liver, bile duct, liver, bile duct, bile duct, liver, bile duct, liver, bile duct, bile duct, liver, liver On the other hand, the antibodies against clonorchiasis were used to extract the interaction between clonorchiasis and clonorchiasis. The enzyme hOGG1 assay was used to analyze the enzyme polymorphisms of other enzymes.

项目成果

Sugihara, E., et al.: "Enhanced expression of MYCN leads to centrosome hyperamplification after DNA damage in neuroblastoma cells."Oncogene. 23. 1005-1009 (2004)

Sugihara, E. 等人：“神经母细胞瘤细胞 DNA 损伤后，MYCN 表达增强会导致中心体过度扩增。”癌基因。

Miyahara, N., et al.: "Expression of UDP-N-acetyl-α-D-galactosamine-polypeptide GaINAc N-acetylgalactosaminyl transferase isozyme 3 in the subserosal layer correlates with postsurgical survival of pathological tumor stage 2 carcinoma of the gallbladder"Cl

Miyahara, N. 等人：“浆膜下层中 UDP-N-乙酰基-α-D-半乳糖胺多肽 GaINAc N-乙酰半乳糖胺基转移酶同工酶 3 的表达与胆囊病理肿瘤 2 期癌的术后生存相关”氯

Ghosh, M., et al.: "Establishment and characterization of unique human gallbladder cancer cell lines"Int.J.Oncology. (In press). (2004)

Ghosh, M. 等人：“独特的人胆囊癌细胞系的建立和表征”Int.J.Oncology。

Lieng swangwong, U., et al.: "Infrequent microsatellite instability in liver fluke infection-associated intrahepatic cholangiocarcinomas from Thailand"Int.J.Cancer.. 107. 375-380 (2003)

Lieng swangwong, U., et al.：“来自泰国的肝吸虫感染相关肝内胆管癌中罕见的微卫星不稳定性”Int.J.Cancer.. 107. 375-380 (2003)

Honjo, S., et al.: "Neuroblastoma trends in Osaka, Japan and Great Britain 1970-1994, in relation to screening"Int.J.Cancer. 103. 538-543 (2003)

Honjo, S. 等人：“1970-1994 年日本大阪和英国的神经母细胞瘤趋势与筛查的关系”Int.J.Cancer。