Assessment of the efficacy of collagen-coated-bead columns for antiplatelet therapy in clinical settings

评估临床环境中胶原蛋白涂层珠柱抗血小板治疗的功效

• 批准号: 21790532
• 负责人: KANEKO Makoto
• 金额: $ 2.33万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790532/
• 关键词: 血小板機能検査; 抗血小板薬; コラーゲンビーズカラム; 薬効モニタリング; 抗血小板剤

In this study, we evaluated the utility of 2 types of collagen-coated-bead columns in the assessment of platelet function associated with the efficacy of antiplatelet drug therapy and prediction of bleeding. In particular, platelet activity was measured to diagnose whether patients with thrombotic disease were drug resistant and to assess patients who were or were not administered antiplatelet agents before endoscopic biopsy.Large variation in the platelet retention rate among healthy individuals was observed using the collagen-coated-bead column; this may reflect individual differences but not methodological variability. Overlapping of the retention rates of patients who were and were not administered the antiplatelet reagents may limit the establishment of reference intervals for platelet function in the present assay system. However, the platelet retention rate may reflect individual variability in response to the antiplatelet drugs and may be useful for monitoring the efficacy of antiplatelet therapy in the same individual. Thus, it is important to evaluate platelet function before and after medication for monitoring the effects of antiplatelet drugs. We also evaluated platelet function by using light-transmission platelet aggregometer and whole-blood aggregometer with a screen filtration pressure method. Platelet function assays are not interchangeable and may vary on the basis of the assay used. These findings suggest that the platelet function assay needs to be used in suitable methods and to be evaluated for additional verification of its clinical utility.

在这项研究中，我们评估了两种类型的胶原蛋白涂层珠柱在评估血小板功能与抗血小板药物治疗的疗效和预测出血中的效用。特别是，血小板活性测定，以诊断是否有血栓性疾病的患者耐药，并评估患者谁是或不给予抗血小板药物内镜活检前，血小板留存率在健康个体之间的大的变化，观察使用胶原包被的珠柱，这可能反映了个体差异，但不是方法的变异性。接受和未接受抗血小板试剂治疗的患者的保留率重叠可能会限制本检测系统中血小板功能参考区间的建立。然而，血小板留存率可以反映个体对抗血小板药物反应的差异性，并可用于监测同一个体抗血小板治疗的疗效。因此，在用药前后进行血小板功能的检测对于监测抗血小板药物的疗效具有重要意义。我们还通过使用透光血小板聚集仪和全血聚集仪与筛网过滤压力法来评价血小板功能。血小板功能测定不可互换，可能因所用测定而异。这些结果表明，血小板功能测定需要使用合适的方法，并进行评价，以进一步验证其临床效用。

项目成果

血小板・凝固検査

血小板/凝血测试

• 发表时间: 2011
• 作者: 金子誠;矢冨裕;金子誠
• 通讯作者: 金子誠

血液領域におけるピットフォール 血小板凝集能検査法の標準化 現状と問題点

血液领域的陷阱 血小板聚集检测方法标准化现状及问题

• 发表时间: 2009
• 期刊: 日本臨床検査自動化学会会誌
• 作者: 金子誠;佐藤金夫;尾崎由基男
• 通讯作者: 尾崎由基男

抗血小板薬に対する反応多様性

抗血小板药物反应的多样性

• 发表时间: 2011
• 期刊: 日本検査血液学会雑誌
• 作者: Ono S;Fujishiro M;Kodashima S;Takahashi Y;Minatsuki C;Mikami-Matsuda R;Asada-Hirayama I;Konno-Shimizu M;Tsuji Y;Mochizuki S;Niimi K;Yamamichi N;Kaneko M;Yatomi Y;Koike K;金子誠
• 通讯作者: 金子誠

Evaluation of safety of endoscopic biopsy without cessation of antithrombotic agents in Japan

• DOI: 10.1007/s00535-012-0538-7
• 发表时间: 2012-07-01
• 期刊: JOURNAL OF GASTROENTEROLOGY
• 影响因子: 6.3
• 作者: Ono, Satoshi;Fujishiro, Mitsuhiro;Koike, Kazuhiko
• 通讯作者: Koike, Kazuhiko

血小板関連疾患 血小板機能異常症の診断と対応

血小板相关疾病 血小板功能障碍的诊断与治疗

• 发表时间: 2009
• 期刊: 日本血栓止血学会誌
• 作者: 金子誠;矢冨裕
• 通讯作者: 矢冨裕