Effect of one-lung ventilation on acute lung injury

本研究的目的是探讨单肺通气（OLV）对急性肺损伤（ALI）的影响，特别是与肺衰竭后再充气的关系。本实验采用全身麻醉大鼠。OLV组60min， OLV组60min后行双肺通气（DLV）。仅行DLV 120min（对照组）。对照组未见破坏性变化。OLV组肺细胞壁破坏，炎性细胞迁移，肺出血。在OLV组中，充气肺的这种损伤变化比通气肺明显。OLV组大鼠血浆及支气管肺泡灌洗液（BALF）中炎症因子（TNF-α、IL-1β、IL-6、IL-8）、HMGB1浓度均高于对照组。特别是在OLV组，放气肺BALF中HMGB1浓度高于通气肺。综上所述，OLV术后ALI有可能是由肺萎陷作用和通气肺损伤所致。

The aim of this study was to investigate the effect of one-lung ventilation(OLV) on acute lung injury(ALI), especially in relation to re-inflated after deflated lung. This experiment involved general anesthetized rats. OLV was performed for 60min, and then double-lung ventilation(DLV) was performed 60min(OLV group). Only DLV was performed for 120min(control group). No destructive change was observed in the control group. On the contrary, destruction of pulmonary cell walls, migration of inflammatory cells, and pulmonary hemorrhages were present in OLV group. In OLV group, such damaged change in deflated lung was more remarkable than in ventilated lung. Inflammatory cytokine(TNF-α、IL-1β、IL-6、IL-8), HMGB1 concentrations in both plasma and bronchoalveolar lavage fluid(BALF) in OLV group tended to be higher than in control group. Particularly in OLV group, HMGB1 concentration in BALF in deflated lung was higher than in ventilated lung. In conclusion, ALI following the OLV had possibility to caused by effect of deflated lung as well as injury of ventilated lung.