Analysis of wheat protein in sera from patients with wheat allergy using mass spectrometer

使用质谱仪分析小麦过敏患者血清中的小麦蛋白

• 批准号: 22790930
• 负责人: TAKAHASHI Hitoshi
• 金额: $ 2.41万
• 依托单位: Shimane University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790930/
• 关键词: 食物アレルギー; 抗原; IgE; 質量分析

The aims of this study were to identify wheat proteins in sera of patient with wheat dependent exercise-induced anaphylaxis (WDEIA) during provocation tests using iTRAQ-based analysis, and to evaluate usefulness of CAP-fluorescent enzyme-immunoassay (CAP-FEIA) using recombinant high molecular weight-glutein. In iTRAQ-based analysis, although wheat proteins were detected in patients’ sera, those wheat proteins were not involving with allergic reactions. When recombinant HMW-glutenin was immobilized to CAP-FEIA, it was confirmed that detection of specific IgE anaginst recombinant HMW-glutenin is useful for diagnosis of WDEIA when combined with the CAP-FEIA (recombinant omega-5 gliadin) test.

本研究的目的是利用itraq分析在激发试验中鉴定小麦依赖性运动诱发性过敏反应（WDEIA）患者血清中的小麦蛋白，并利用重组高分子量谷蛋白评价cap -荧光酶免疫测定（CAP-FEIA）的有效性。在基于itraq的分析中，虽然在患者血清中检测到小麦蛋白，但这些小麦蛋白与过敏反应无关。将重组hmw -谷蛋白固定在CAP-FEIA上，与CAP-FEIA（重组omega-5麦胶蛋白）试验联合检测重组hmw -谷蛋白特异性IgE抗体，有助于诊断WDEIA。

项目成果

Recombinant high molecular weight-glutenin subunit-specific IgE detection is useful in identifying wheat-dependent exercise-induced anaphylaxis complementary to recombinant omega-5 gliadin-specific IgE test

• DOI: 10.1111/j.1365-2222.2012.04039.x
• 发表时间: 2012-08-01
• 期刊: CLINICAL AND EXPERIMENTAL ALLERGY
• 影响因子: 6.1
• 作者: Takahashi, H.;Matsuo, H.;Morita, E.
• 通讯作者: Morita, E.

小麦水溶性アレルゲンの大腸菌における可溶化発現.

小麦水溶性过敏原在大肠杆菌中的可溶性表达。

• 发表时间: 2012
• 作者: 松山梓;横大路智治; 高橋仁;森田栄伸;松尾裕彰.
• 通讯作者: 松尾裕彰.

牛肉アレルギーの抗原の同定とセツキシマブとの交叉反応性.

鉴定与牛肉过敏相关的抗原以及与西妥昔单抗的交叉反应性。

• 发表时间: 2012
• 作者: 高橋 仁;千貫祐子;森田栄伸.
• 通讯作者: 森田栄伸.

Higher allergenicity of high molecular weight hydrolysed wheat protein in cosmetics for percutaneous sensitization

• DOI: 10.1111/j.1600-0536.2012.02168.x
• 发表时间: 2013-02-01
• 期刊: CONTACT DERMATITIS
• 影响因子: 5.5
• 作者: Chinuki, Yuko;Takahashi, Hitoshi;Morita, Eishin
• 通讯作者: Morita, Eishin

Prevalence of Wheat Allergy in Japanese Adults

日本成年人小麦过敏的患病率

• DOI: 10.2332/allergolint.11-oa-0345
• 发表时间: 2012
• 期刊: Allergology International
• 影响因子: 6.8
• 作者: Morita E;Chinuki Y;Takahashi H;Nabika T;Yamasaki M;Shiwaku K.
• 通讯作者: Shiwaku K.