Analysis of wheat protein in sera from patients with wheat allergy using mass spectrometer

本研究的目的是确定小麦依赖性运动过敏反应（WDEIA）患者血清中的小麦蛋白在激发试验中使用iTRAQ为基础的分析，并评估使用重组高分子量谷蛋白的CAP-荧光酶免疫分析（CAP-FEIA）的有用性。在iTRAQ分析中，虽然在患者血清中检测到小麦蛋白，但这些小麦蛋白不涉及过敏反应。当重组HMW-麦谷蛋白被固定到CAP-FEIA上时，证实了当与CAP-FEIA（重组ω-5麦醇溶蛋白）测试组合时，特异性IgE拮抗剂重组HMW-麦谷蛋白的检测对于WDEIA的诊断是有用的。

The aims of this study were to identify wheat proteins in sera of patient with wheat dependent exercise-induced anaphylaxis (WDEIA) during provocation tests using iTRAQ-based analysis, and to evaluate usefulness of CAP-fluorescent enzyme-immunoassay (CAP-FEIA) using recombinant high molecular weight-glutein. In iTRAQ-based analysis, although wheat proteins were detected in patients’ sera, those wheat proteins were not involving with allergic reactions. When recombinant HMW-glutenin was immobilized to CAP-FEIA, it was confirmed that detection of specific IgE anaginst recombinant HMW-glutenin is useful for diagnosis of WDEIA when combined with the CAP-FEIA (recombinant omega-5 gliadin) test.