Analysis of wheat protein in sera from patients with wheat allergy using mass spectrometer

本研究的目的是利用itraq分析在激发试验中鉴定小麦依赖性运动诱发性过敏反应（WDEIA）患者血清中的小麦蛋白，并利用重组高分子量谷蛋白评价cap -荧光酶免疫测定（CAP-FEIA）的有效性。在基于itraq的分析中，虽然在患者血清中检测到小麦蛋白，但这些小麦蛋白与过敏反应无关。将重组hmw -谷蛋白固定在CAP-FEIA上，与CAP-FEIA（重组omega-5麦胶蛋白）试验联合检测重组hmw -谷蛋白特异性IgE抗体，有助于诊断WDEIA。

The aims of this study were to identify wheat proteins in sera of patient with wheat dependent exercise-induced anaphylaxis (WDEIA) during provocation tests using iTRAQ-based analysis, and to evaluate usefulness of CAP-fluorescent enzyme-immunoassay (CAP-FEIA) using recombinant high molecular weight-glutein. In iTRAQ-based analysis, although wheat proteins were detected in patients’ sera, those wheat proteins were not involving with allergic reactions. When recombinant HMW-glutenin was immobilized to CAP-FEIA, it was confirmed that detection of specific IgE anaginst recombinant HMW-glutenin is useful for diagnosis of WDEIA when combined with the CAP-FEIA (recombinant omega-5 gliadin) test.