STUDIES OF PH-ZONE REFINING COUNTERCURRENT CHROMATOGRAPHY

PH区精炼逆流色谱研究

• 批准号: 3757624
• 负责人: Y ITO
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3757624
• 关键词: acidity /alkalinity; aminoacid; chemical models; dyes; ion exchange chromatography; model design /development; oligopeptides; plant extracts; vinca alkaloids

Our continuous research on pH-zone-refining CCC has extended the domain of its applications which include: (1) separation of basic compounds using triethylamine as a retainer base, (2) the development of a displacement operation mode which may be applied to a ligand-affinity separation, and (3) the separation of chiral compounds using a chiral selector in the stationary phase. Utility of the method has been demonstrated on the separation and purification of various analytes such as alkaloids from extracts of Amaryllis and Vinca Minor, structural isomers from a reaction mixture, the acidic and basic derivatives of amino acids and oligopeptides and a pair of optical isomers of D,L- leucine derivatives.

我们对ph区精炼CCC的持续研究拓展了研究领域