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PREPARATIVE HIGH-SPEED COUNTERCURRENT CHROMATOGRAPH WITH THREE MULTILAYER COILS

PREPARATIVE HIGH-SPEED COUNTERCURRENT CHROMATOGRAPH WITH THREE MULTILAYER COILS
具有三个多层线圈的制备型高速逆流色谱仪
批准号：
3858002
负责人：
Y ITO
金额：
$0.0万
依托单位：
NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3858002
关键词：
acetates aminoacid analog bioengineering /biomedical engineering butanols centrifugation dipeptides glycine molecular polarity nonwater solvent solvent extraction solvents tyrosine analog water

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中文摘要
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英文摘要
The capability of the high speed countercurrent chromatograph was evaluated in separation of polar dipeptides with a new set of multilayer coils of a small helical diameter. Using a hydrophilic two-phase solvent system composed of n-butanol, acetic acid and water at 4:1:5 volume ratio, four components (L-tyrosylglycine, L-valyl-L-tyrosine, L-leucyl-L-tyrosine and L-tryptophyl-L-tyrosine) were separated at a flow rate of 1 to 2 ml/min. Best results were obtained by eluting the upper nonaqueous phase in a head to tail direction under 800 rpm of coil rotation. Four components were completely resolved in 5 to 10 hours.
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