PREPARATIVE HIGH-SPEED COUNTERCURRENT CHROMATOGRAPH

制备型高速逆流色谱仪

• 批准号: 3878927
• 负责人: Y ITO
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3878927
• 关键词: centrifugation

A high-speed CCC centrifuge was designed and constructed for performing preparative-scale separations. The apparatus holds three multilayer coils (2.6mm ID) which are connected in series to make up a total capacity of about 1600ml. The maximum speed is 1000 rpm. Preparative capability of the apparatus was demonstrated on the separation of a 4g quantity of DNP amino acids with a two-phase solvent system composed of CHC13/CH3COOH/0.1NEC1 (2:2: 1). Four components were resolved in 7.5h at partition efficiencies ranging from 1000 to 1800 theoretical plates.

设计并建造了一台高速CCC离心机， 规模分离。该装置容纳三个多层线圈 （2.6mm ID），串联起来，总容量为 约1600 ml。最高转速为1000 rpm。的分解能力， 在分离4g量的DNP氨基上展示了装置 酸与由CHCl 3/CH 3COOH/0.1NEC1组成的两相溶剂系统 （2：2：1）。在7.5h的分配效率下，四种组分在7.5h内被分离 范围为1000 - 1800理论塔板。