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SEPARATION OF PROTEINS BY AQUEOUS-AQUEOUS POLYMER PHASE SYSTEMS

SEPARATION OF PROTEINS BY AQUEOUS-AQUEOUS POLYMER PHASE SYSTEMS
通过水-水聚合物相系统分离蛋白质
批准号：
3843284
负责人：
Y ITO
金额：
$0.0万
依托单位：
NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3843284
关键词：
acidity /alkalinity alpha globulin bioengineering /biomedical engineering biomedical equipment development centrifugation dextrans ethylene glycols gamma globulin histones human tissue phosphates polymers potassium protein purification serum albumin solvents water solution

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中文摘要
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英文摘要
A new model of the cross-axis synchronous flow-through coil planet centrifuge was designed and fabricated at the NIH machine shop. The apparatus holds a pair of multilayer coil separation columns one on each side of the rotary frame at an off-center position. 3.8cm from the centrifuge axis and 13.5cm from the midpoint of the rotary shaft. The capability of the apparatus was evaluated in terms of retention of the stationary phase and partition efficiency using aqueous-aqueous polymer phase systems composed of dextran T500 and polyethylene glycol 8000 where the pH of the solvent was adjusted by 10mM potassium phosphate. The results indicated that the present system can hold a stationary phase at 45% of the total column capacity at 900 rpm and at a flow rate of 1ml/min. The performance of the apparatus was demonstrated in separation of various stable proteins including human serum albumin, alpha and gamma globulins, and histones.
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COUNTERCURRENT CHROMATOGRAPHY FOR SEPARATION OF POLAR COMPOUNDS
FOAM COUNTERCURRENT CHROMATOGRAPHY WITH A COIL PLANET CENTRIFUGE
ANALYTIC HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY WITH A COIL PLANET CENTRIFUGE
STUDIES OF PH ZONE REFINING COUNTERCURRENT CHROMATOGRAPHY