ANALYTIC HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY WITH A COIL PLANET CENTRIFUGE

分析高速逆流色谱(HSCCC) 成功应用于各种天然产物的分离 其中包括沙棘中的黄酮类成分，沙棘中的生物碱 粉防己碱，羟基蒽醌衍生物 大黄等。结果表明： 1.有效分离原油中存在的多种组分 可以在短时间内完成提取，方法是使用 连续的正常和反向洗脱模式 中断离心机运转。 2.使用氯仿溶剂系统，它提供了大量的 两相之间的密度不同，流速可以是 最高可达5毫升/分钟而不影响峰值分辨率，因此 在不到15分钟内实现高效分离，如 分析型高效液相色谱仪。 3.与LC/MS系统类似，HSCCC可以有效地 使用热喷雾毛细管与质谱仪对接 管子装置。

Analytic high-speed countercurrent chromatography (HSCCC) was successfully applied to separations of various natural products which include flavonoids from sea buckthorn, alkaloids from Stephania tetrandra S. Moore, hydroxyanthraquinone derivatives from rheum, etc. The results indicated: 1. Efficient separations of multiple components present in a crude extract can be effected in a short period of time by using the normal and reversed elution modes in succession without interrupting the centrifuge run. 2. With chloroform solvent systems, which provide a large difference in density between the two phases, the flow rate can be increased up to 5 ml/min without affecting peak resolution, thus achieving efficient separations in less than 15 minutes as in analytical HPLC. 3. In analogy to the LC/MS system, HSCCC can be effectively interfaced with a mass spectrometer using a thermospray capillary tube device.