ANALYTIC HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY WITH A COIL PLANET CENTRIFUGE

带盘管行星离心机的分析高速逆流色谱

• 批准号: 3920044
• 负责人: Y ITO
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3920044
• 关键词: alkaloids; angiosperms; centrifugation; chloroform; flavonoids; hydroquinones; indoles; mass spectrometry; plant extracts; solvents

Analytic high-speed countercurrent chromatography (HSCCC) was successfully applied to separations of various natural products which include flavonoids from sea buckthorn, alkaloids from Stephania tetrandra S. Moore, hydroxyanthraquinone derivatives from rheum, etc. The results indicated: 1. Efficient separations of multiple components present in a crude extract can be effected in a short period of time by using the normal and reversed elution modes in succession without interrupting the centrifuge run. 2. With chloroform solvent systems, which provide a large difference in density between the two phases, the flow rate can be increased up to 5 ml/min without affecting peak resolution, thus achieving efficient separations in less than 15 minutes as in analytical HPLC. 3. In analogy to the LC/MS system, HSCCC can be effectively interfaced with a mass spectrometer using a thermospray capillary tube device.

分析型高速逆流色谱（HSCCC） 成功应用于各种天然产物的分离 包括沙棘中的黄酮类化合物， 粉防己摩尔，羟基蒽醌衍生物， 大黄等。结果表明： 1. 有效分离原油中的多种组分 提取可以在短时间内通过使用 连续的正常和反向洗脱模式， 中断离心机运行。 2. 与氯仿溶剂系统，这提供了一个大的 由于两相之间的密度差，流速可以是 增加至5 ml/min，而不影响峰分辨率，因此 在不到15分钟的时间内实现高效分离， 分析型HPLC。 3. 与LC/MS系统类似，HSCCC可以有效地 使用热喷雾毛细管与质谱仪连接 管装置。