COUNTERCURRENT CHROMATOGRAPHY FOR SEPARATION OF POLAR COMPOUNDS

对极性化合物(Trp-Leu和Val-Tyr)进行了克量分离。 用由正丁醇醋酸酯组成的亲水溶剂体系实现的 酸/水(4：1：5)，使用大直径盘管柱(0.55 cm) (身份证)在水平流过盘管行星离心机上 上一份报告。使用短螺旋色谱柱，固定相保留 和峰分辨率在不同操作条件下进行了研究 改变流动相的螺旋直径、转速、流速和洗脱模式 相位。在最佳操作条件下(300rpm和120ml/h流量 速率)制备规模的分离在1g的量上进行 长柱(30米长，750毫升容量，7.5厘米)样品 螺旋直径)。本系统的载样能力可 通过使用更长和/或更大直径的柱子来进一步增加。

Gram quantity separation of polar compounds (trp-leu and val-tyr) was achieved with a hydrophilic solvent system composed of n-butanol acetic acid/water (4:1:5) by the use of a large diameter coiled column (0.55 cm i.d.) on a horizontal flow-through coil planet centrifuge described in the previous report. Using a short coiled column, stationary phase retention and peak resolution were studied under various operational conditions by altering helical diameter, rpm, flow rates, and elution mode of the mobile phase. Under the optimum operational conditions (300 rpm and 120 ml/h flow rate) preparative-scale separations were performed on 1g quantity of samples with a long column (30 m in length, 750 ml capacity and 7.5 cm helical diameter). The sample-loading capacity of the present system may be further increased by the use of longer and/or larger-diameter columns.