COUNTERCURRENT CHROMATOGRAPHY FOR SEPARATION OF POLAR COMPOUNDS

用于分离极性化合物的逆流色谱

• 批准号: 4694537
• 负责人: Y ITO
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4694537
• 关键词: 2,4 dinitrophenol; aminoacid; centrifugation; fluidity; peptides; purines; pyrimidines; solvents; synthetic peptide

Gram quantity separation of polar compounds (trp-leu and val-tyr) was achieved with a hydrophilic solvent system composed of n-butanol acetic acid/water (4:1:5) by the use of a large diameter coiled column (0.55 cm i.d.) on a horizontal flow-through coil planet centrifuge described in the previous report. Using a short coiled column, stationary phase retention and peak resolution were studied under various operational conditions by altering helical diameter, rpm, flow rates, and elution mode of the mobile phase. Under the optimum operational conditions (300 rpm and 120 ml/h flow rate) preparative-scale separations were performed on 1g quantity of samples with a long column (30 m in length, 750 ml capacity and 7.5 cm helical diameter). The sample-loading capacity of the present system may be further increased by the use of longer and/or larger-diameter columns.

对极性化合物色氨酸-亮氨酸和缬氨酸-酪氨酸进行了定量分离， 用由乙酸正丁醇组成的亲水性溶剂系统实现 酸/水（4：1：5）通过使用大直径盘绕柱（0.55cm （同上）在美国专利No.2005，000中所述的水平流通盘管行星式离心机上， 以前的报告。 使用短螺旋柱，固定相保留 和峰分离度进行了研究，在各种操作条件下， 改变移动的的螺旋直径、rpm、流速和洗脱模式 相位 在最佳操作条件（300 rpm和120 ml/h流量）下， 速率）对1g量的 用长柱（30 m长，750 ml容量和7.5 cm 螺旋直径）。 本系统的样品装载容量可以 通过使用更长和/或更大直径的柱进一步增加。