HORIZONTAL FLOW-THROUGH COIL PLANET CENTRIFUGE WITH MULTILAYER COIL SET

带多层线圈组的卧式流通线圈行星离心机

• 批准号: 3899182
• 负责人: Y ITO
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3899182
• 关键词: centrifugation

The apparatus holds a set of four identical multilayer coils around the column holder. These columns are interconnected in series to make up a total capacity of about 200ml. Because of the eccentric orientation of the multilayer coils with respect to the axis of the holder, the radially acting centrifugal force from the holder axis retains the stationary phase, either the lighter or the heavier phase, in each helical turn of the coil while the planetary motion of the coil produces efficient mixing of the two solvent phases. The system provides universal application of two-phase solvent systems including aqueous-aqueous polymer phase systems used for partition of macromolecules and cell particles. The capability of the apparatus was successfully demonstrated in separation of cytochrome c and lysozyme in a polymer phase system composed of 12.5% polyethylene glycol 1000 in IM potassium phosphate aqueous solution.

该装置将一组四个相同的多层线圈绕在 柱固定器。这些柱子串联在一起，组成了一个 总容量约为200毫升。因为它的偏心方向 多层线圈相对于夹持器的轴线，径向 来自夹持器轴的作用离心力保持静止相， 在线圈的每一次螺旋回转中，要么是较轻的阶段，要么是较重的阶段 而线圈的行星运动产生了两者的有效混合 溶剂相。该系统提供了两相的普遍应用 溶剂体系，包括水-水聚合物相体系，用于 大分子和细胞颗粒的分离。该系统的能力 成功实现了细胞色素c和细胞色素c的分离。 12.5%聚乙二醇聚合相体系中的溶菌酶 1000在IM磷酸二氢钾水溶液中。