EVALUATION OF ANALYTICAL COUNTERCURRENT CHROMATOGRAPHS

两种高速逆流色谱模型的性能 逆流色谱仪(HSCCC-4000)和分析环形线圈 离心机(TCC)，根据理论板数进行评估， 分离因子和分离时间来评估它们的分析 能力。进行了一系列实验，以研究 盘管柱内径、长度及流量的影响 流动相在两相溶剂中分离吲哚生长素的研究 不同温度下正己烷/乙酸乙酯/甲醇/水组成的体系 体积比。吲哚生长素的三种成分完全是 配备多层线圈的HSCCC系统可在16分钟内解决问题 0.55 mm直径的聚四氟乙烯管，理论板数从829到1,290。 在配备了TCC系统的情况下，24分钟内实现了类似的分离 直径为0.3 mm的聚四氟乙烯管，理论板数从969到1,811。它 结论是，这两个系统在 目前的发展阶段。

Performance of two countercurrent chromatographic models, high-speed countercurrent chromatograph (HSCCC-4000) and analytical toroidal coil centrifuge (TCC), is evaluated in terms of theoretical plate number, resolution factor and separation time to assess their analytical capability. A series of experiments was conducted to investigate the effects of internal diameter and length of the coiled column and flow rate of the mobile phase on the separation of indole auxins in two-phase solvent systems composed of n-hexane/ethyl acetate/methanol/water at different volume ratios. The three components of the indole auxins were completely resolved in 16 min with the HSCCC system equipped with a multilayer coil of a 0.55 mm ID PTFE tube with theoretical plates ranging from 829 to 1,290. Similar separation was achieved in 24 min with the TCC system equipped with a 0.3mm ID PTFE tube with theoretical plates ranging from 969 to 1,811. It is concluded that both systems have comparable analytical capability at the present stage of development.