DEVELOPMENT OF FOAM COUNTERCURRENT CHROMATOGRAPHY

提出了一种新的泡沫分离方法。 的 方法使用气液双逆流通过螺旋柱 受到特定类型的同步行星运动的影响。 样品 在塔的中间部分引入，以分批或 连续模式，根据泡沫亲和力分离。 任何 对泡沫具有亲和力的材料随着发泡而快速地被携带 流并通过柱的一端洗脱，而其它材料 在相反的方向上被液流携带并洗脱出来 穿过柱子的另一端 该泡沫的性能 逆流色谱法是证明分离的 罗丹明B和伊文思蓝与阴离子表面活性剂SDS作为捕收剂 罗丹明B。 成功初步分离蛋白质样品，BSA 和绵羊血红蛋白，表明本方法可以有效地 适用于各种生物样品的分离和纯化， 如酶、膜受体等。

A novel method of performing foam separation has been developed. The method uses a gas-liquid dual countercurrent flow through a helical column subjected to a particular type of synchronous planetary motion. Samples introduced at the middle portion of the column, in either batch or continuous mode, are separated according to the foam affinity. Any material having an affinity to the foam is quickly carried with the foaming stream and eluted through one end of the column whereas other materials are carried with the liquid stream in the opposite direction and eluted out through the other end of the column. Capability of this foam countercurrent chromatographic method is demonstrated on separations of rhodamine B and Evans blue with an anionic surfactant, SDS, as a collector of rhodamine B. Successful preliminary separation of protein samples, BSA and sheep hemoglobin, indicates that the present method may be effectively applied to separation and purification of various biological samples such as enzymes, membrane receptors, etc.