FOAM COUNTERCURRENT CHROMATOGRAPHY WITH A COIL PLANET CENTRIFUGE

带盘管行星离心机的泡沫逆流色谱

• 批准号: 3899183
• 负责人: Y ITO
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3899183
• 关键词: body fluids; centrifugation; hydropathy

By the use of the coil planet centrifuge reported earlier (Z0I HL 0145502 LTD) foam countercurrent chromatography (CCC) was successfully applied to separation of bacitracin components with nitrogen gas and distilled water free of surfactant or other additives. In the batch foam separation, the bacitracin components were separated according to their hydrophobicity. In continuous enrichment of the diluted sample solution (50 ppm), the foam fraction showed over 1000 to 2000 fold enrichment of hydrophobic components such as bacitracin A and F while in the liquid fraction no hydrophobic components were eluted. The method may be extremely useful in enrichment of bioactive minor components in biological fluids such as urine, blood and blood dialysate, cell culture medium-, etc.

通过使用早先报告的螺旋行星离心机(Z0I HL 0145502 LTD)泡沫逆流色谱(CCC)成功地应用于 氮气和蒸馏水分离杆菌肽组分 不含表面活性剂或其他添加剂。在间歇泡沫分离中， 根据芽孢杆菌毒素的疏水性对其进行分离。在……里面 连续浓缩稀释的样品溶液(50ppm)，泡沫 组分显示疏水成分的浓缩超过1000到2000倍 例如杆菌素A和F，而在液体组份中没有疏水性 组分被洗脱。该方法在富集铀方面可能非常有用。 生物体液中具有生物活性的次要成分，如尿液、血液和 血液透析液、细胞培养液等。

项目成果

Restoring mitochondrial DNA copy number preserves mitochondrial function and delays vascular aging in mice.

• DOI: 10.1111/acel.12773
• 发表时间: 2018-08
• 期刊: Aging cell
• 影响因子: 7.8
• 作者: Foote K;Reinhold J;Yu EPK;Figg NL;Finigan A;Murphy MP;Bennett MR
• 通讯作者: Bennett MR