CROSS-AXIS SYNCHRONOUS FLOW-THROUGH COIL PLANET CENTRIFUGE

一种改进的十字轴同步通流线圈行星模型 前面描述的离心机(Z01 HL 01462-06 LBC)已经过测试 蛋白质分离，在分析和制备规模，使用一个 聚乙二醇1000(PEG1000)和聚乙二醇1000(PEG1000)组成的两相聚合物体系 磷酸二氢钾，每个12.5%(w/w)。四个人的混合物 稳定的蛋白质包括细胞色素c、肌红蛋白、卵清蛋白和 在两个色谱柱中以260的效率分离血红蛋白 至670个理论板(TP)。进一步的研究表明，这 蛋白质分离效率相对较低可能是由于 蛋白质分子的大小和/或异质性而不是很高 聚合物相体系的粘度。目前的制度一直是 应用于大肠杆菌蛋白质的分级分离，取得了良好的效果。

An improved model of the cross-axis synchronous flow-through coil planet centrifuge previously described (Z01 HL 01462-06 LBC) has been tested for protein separations, in both analytical and preparative scale, using a two-phase polymer system composed of polyethylene glycol 1000 (PEG) and dibasic potassium phosphate each at 12.5% (w/w). A mixture of four stable proteins including cytochrome c, myoglobin, ovalbumin and hemoglobin were resolved in both columns at efficiencies ranging from 260 to 670 theoretical plates (TP). Further studies suggested that this relatively low efficiency of the protein separation may be due to the size and/or heterogeneity of the protein molecules rather than high viscosity of the polymer phase system. The present system has been applied to fractionation of E. coli proteins with promising results.