THEORETICAL STUDIES ON PH-ZONE REFINING COUNTERCURRENT CHROMATOGRAPHY

PH区精炼逆流色谱理论研究

• 批准号: 3779528
• 负责人: Y ITO
• 金额: --
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3779528
• 关键词: acidity /alkalinity; aminoacid; biophysics

A series of basic experiments has been performed with a set of DNP-amino acids and two-phase solvent system composed of methyl tertiary-butyl ether/water. The retainer acid (TFA) was added to the stationary phase and the eluent base (NH3) to the mobil phase at various concentrations. The results were found to be consistent with the mathematical analyses developed on the hydrodynamic mechanism operating in the present system. The overall results of the studies revealed that the present method bears a close resemblance to displacement chromatography in many aspects: One solute suppresses the ionization or affinity of others to alter the partition coefficient of the latter; in the column a train of sharply defined solute bands is formed and each band moves together at the same rate; impurities are concentrated at the boundaries of each solute bands; etc. However, there is a contrasting difference in the action of the key reagents. In pH-zone-refining CCC, the retainer acid transfers solute molecules from the mobile phase to the stationary phase at the front end of the solute bands whereas in displacement chromatography the displacer displaces solute molecules absorbed onto the stationary phase at the rear end of the solute bands. These mutually reversed actions of the key reagents at the opposite ends of the solute bands produce identical elution profiles. From this viewpoint, the present technique may also be called "Reverse Displacement CCC". This term further suggests the presence of displacement CCC as well as that of reverse displacement chromatography.

用一组DNP-amino进行了一系列基础实验， 酸和由甲基叔丁基 乙醚/水。 在固定相中加入固定酸（TFA）， 和洗脱剂碱（NH3）以各种浓度加入到流动相中。 计算结果与数学分析相一致 在本系统中操作的流体动力学机制上开发的。 研究的总体结果表明，本方法具有 在许多方面与置换色谱法非常相似： 溶质抑制其他溶质的电离或亲和力， 后者的分配系数;在列中， 形成确定的溶质带，并且每个带同时移动到一起。 速率;杂质集中在每个溶质带的边界; 等 然而，有一个对比的差异，在行动的关键 试剂 在pH区带精炼CCC中，保留酸传递溶质 在前端从移动的相到固定相的分子 而在置换色谱中，置换剂 置换吸附在后部固定相上的溶质分子 溶质带的末端。 钥匙的这些相互反转的动作 在溶质带的相对端的试剂产生相同的 洗脱曲线。 从这个观点来看，本技术还可以 被称为“反向位移CCC”。 这一术语进一步表明， 存在位移CCC以及反向位移 层析

项目成果

Heterogeneity of T-Tubules in Pig Hearts.

• DOI: 10.1371/journal.pone.0156862
• 发表时间: 2016
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Gadeberg HC;Bond RC;Kong CH;Chanoit GP;Ascione R;Cannell MB;James AF
• 通讯作者: James AF