ASSESSMENT OF THE SIZE OF THE LEAK INDUCED IN RETINAL VESSELS USING PITC-DEXTRANS

使用 PITC-DEXTRANS 评估视网膜血管渗漏的大小

• 批准号: 3941671
• 负责人: A G PALESTINE
• 金额: --
• 依托单位: NATIONAL EYE INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3941671
• 关键词: dextrans; disease /disorder model; eye disorder diagnosis; fluorescein angiography; retina circulation disorder; uveitis

Uveitis was induced in two monkeys by immunization with IRBP and serial fluorescein angiograms performed using different sized dextrans linked to fluorescein. The aim of these studies is to provide data on the retinal vessels and toxicology data to enable these agents to be used in humans. We have demonstrated that the larger molecular weight dextrans are less permeable than sodium fluorescein in the inflamed retina. The sites of large molecule leakage show ultrastructural evidence of open tight junctions whereas the areas that leak only sodium fluorescein have closed tight junctions.

用IRBP免疫两只猴子，诱导葡萄膜炎 和使用不同大小的荧光素血管造影剂进行的系列荧光素血管造影 葡聚糖连接荧光素。 这些研究的目的是 提供视网膜血管数据和毒理学数据， 这些药物将用于人类。 我们已经证明 较大分子量的葡聚糖的渗透性比 在发炎的视网膜中发现了荧光素钠 大型网站 分子渗漏显示了超微结构证据， 而仅泄漏荧光素钠的区域 具有封闭的紧密连接。