LYMPHOCYTE MIGRATION IN EXPERIMENTAL AUTOIMMUNE UVEITIS

实验性自身免疫性葡萄膜炎（EAU）是通过免疫大鼠诱导的 和其他实验动物与S-抗原（可溶性抗原，从 视网膜）在该实验室中作为人类的模型进行研究。 眼内炎症。 这种实验性炎症可以是 使用收获的淋巴细胞从供体大鼠转移到幼稚受体 从脾脏或淋巴结中取出 在从细胞中收获细胞后， 供体和三天的培养与刺激抗原，细胞是 注射到腹腔内，五到七天后， 受体大鼠发生EAU。 这种疾病也可以通过使用 腹膜内或眼内注射辅助性T细胞系。 的 疾病的传播机制尚不清楚。 这项工作使用了 放射性标记的淋巴细胞，以确定这些细胞的命运， 淋巴细胞注射到腹膜腔或血液中后， 葡萄膜炎的发展过程。 该项目的目标是 了解炎症的启动机制，希望这些 机制可以扩展并应用于人类炎症。 到目前为止， 已经确定只有一小部分淋巴结淋巴细胞 注射到腹膜腔中的药物实际上是在 诱导EAU。 一亿个细胞转移到腹膜 大约有5,000人到达眼睛。 更多的到达脾脏， 肝脏和胸腺。 这项工作现在正在扩大到研究 腹膜内或眼内T细胞系的迁移模式。

Experimental autoimmune uveitis (EAU) is induced by immunization of rats and other experimental animals withS-antigens (a soluble antigen from the retina) is being investigated in this laboratory as a model of human intra-ocular inflammation. This experimental inflammation can be transferred from donor rats to naive recipients using lymphocytes harvested from the spleen or lymph nodes. Following harvesting of the cells from the donors and three days in culture with stimulating antigen, the cells are injected into the intra-peritoneal cavity and five to seven days later the recipient rats develop EAU. The disease can also be transferred using a T-helper cell line by intra-peritoneal or intra-ocular injection. The mechanism of transfer of disease in unclear. This work has used radioactively labeled lymphocytes to determine the fate of these lymphocytes after injection into the peritoneal cavity or blood during the process of the development of uveitis. The goal of this project is to understand the initiating mechanisms of inflammation in the hope that these mechanisms can be extended and applied to human inflammations. Thus far we have determined that only a small percentage of the lymph node lymphocytes injected into the peritoneal cavity actually reached the eye during the induction of EAU. Of 100 million cells transferred into the peritoneal cavity, approximately 5,000 reach the eye. Many more reach the spleen, liver and thymus however. The work is now being extended to study the migration patterns of intra-peritoneal or intra-ocular T-cells lines.