LYMPHOCYTE MIGRATION IN EXPERIMENTAL AUTOIMMUNE UVEITIS

实验性自身免疫性葡萄膜炎(EAU)是由大鼠免疫诱发的 和其他实验动物使用S抗原(一种来自 视网膜)正在这个实验室中作为人类的模型进行研究 眼内炎症。这种实验性炎症可能是 用采集的淋巴细胞从供体大鼠转移到幼稚的受体 从脾或淋巴结中取出。在从细胞中收获细胞之后 供者和在刺激抗原培养三天后，细胞是 注射到腹膜腔内，五到七天后 受体大鼠发展为EAU。疾病传播的机制是 不清楚。这项工作使用了放射性标记的淋巴细胞来确定 这些淋巴细胞注射到腹膜腔后的去向 在葡萄膜炎的发展过程中。这样做的目的是 该项目的目的是了解炎症的启动机制。 希望这些机制能够推广应用到人类身上 发炎。到目前为止，我们已经确定只有一小部分 注射到腹膜腔的淋巴细胞实际上到达了眼睛 在EAU的诱导过程中。一亿个细胞被转移到 腹膜腔，大约5,000个到达眼睛。更多的人到达 但脾、肝和胸腺。进一步研究时间进程和 到达发炎眼睛的细胞类型正在进行中，目标是 了解EAU中供者和宿主淋巴细胞之间的关系。

Experimental autoimmune uveitis (EAU) is induced by immunization of rats and other experimental animals with S-antigen (a soluble antigen from the retina) is being investigated in this laboratory as a model of human intra-ocular inflammation. This experimental inflammation can be transferred from donor rats to naive recipients using lymphocytes harvested from the spleen or lymph nodes. Following harvesting of the cells from the donors and three days in culture with stimulating antigen, the cells are injected into the intra-peritoneal cavity and five to seven days later the recipient rats develop EAU. The mechanisms of transfer of disease is unclear. This work has used radioactively labeled lymphocytes to determine the fate of these lymphocytes after injection into the peritoneal cavity and during the process of the development of uveitis. The goal of this project is to understand the initiating mechanisms of inflammation in the hope that these mechanisms can be extended and applied to human inflammations. Thus far we have determined that only a small percentage of the lymphocytes injected into the peritoneal cavity actually reach the eye during the induction of EAU. Of 100 million cells transferred into the peritoneal cavity, approximately 5,000 reach the eye. Many more reach the spleen, liver and thymus however. Further studies of the time course and type of cell reaching the inflammed eye are in progrsss with the goal of understanding the relationship between donor and host lymphocytes in EAU.