USE OF THERMO LTQ-FT-MS INSTRUMENT FOR DRINKING WATER RESEARCH

使用热 LTQ-FT-MS 仪器进行饮用水研究

• 批准号: 7358235
• 负责人: RON ORLANDO
• 金额: $ 0.14万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358235
• 关键词: USE; THERMO; LTQ; FT; MS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to use a bio-assay directed approach to focus identification work on the most toxicologically important drinking water disinfection by-products (DBPs). To this end, drinking water is being collected from full-scale treatment plants that use chlorine, ozone, chlorine dioxide, and chloramines as disinfectants, and this drinking water is being fractionated according to polarity (through the use of different polarity XAD resins, preparatory liquid chromatography columns, and solvent gradients) and molecular size (through the use of ultrafiltration and size exclusion chromatography). Molecular weight fractions to be studied include <1000, 1000-3000, 3000-5000, 5000-10,000, and >10,000 Da. The bioassays used include a mammalian cell genotoxicity/cytotoxicity assay and a transgenic medaka fish genotoxicity assay. Gas chromatography with high resolution mass spectrometry is being used to identify the lower molecular weight, non-polar drinking water DBPs, and electrospray ionization (ESI)-MS and ESI-MS/MS is being used to identify high molecular weight and highly polar DBPs.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。该项目的目标是使用生物测定法，将鉴定工作集中在毒理学上最重要的饮用水消毒副产物（DBPs）上。为此，从使用氯、臭氧、二氧化氯和氯胺作为消毒剂的全面处理厂收集饮用水，并根据极性（通过使用不同极性的XAD树脂、制备液相色谱柱和溶剂梯度）和分子大小（通过使用超滤和尺寸排阻色谱法）对饮用水进行分级。待研究的分子量组分包括<1000、1000- 3000、3000-5000、5000- 10，000和> 10，000 Da。所用的生物测定包括哺乳动物细胞遗传毒性/细胞毒性测定和转基因青鳉鱼遗传毒性测定。采用气相色谱法和高分辨率质谱法鉴别低分子量、非极性饮用水消毒副产物，采用电喷雾电离（ESI）-MS和ESI-MS/MS鉴别高分子量和高极性消毒副产物。