LSU VETERINARY COBRE: TNPRC NON-HUMAN PRIMATE CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 非人灵长类动物和实验室核心的目标是为项目负责人和个体研究者提供高度集成的临床管理和实验室研究。 IACUC对非人灵长类动物和实验室核心支持的每个子项目单独进行批准。 非人类灵长类动物和实验室核心购买，房屋和照顾猕猴在这个项目中使用。 核心负责动物的日常临床护理和实验动物程序，如体格检查、病毒接种、血液采集、肠道活检和淋巴结活检。 核心的实验室部分对外周血进行血液学和临床化学分析，流式细胞术用于定量淋巴细胞类型（CD 3、CD 4、CD 8）和免疫学测定，包括使用四聚体技术和细胞内细胞因子染色定量CD 8 T细胞应答。 核心还对这些研究中使用的动物进行常规细菌培养、SIV分离和定量以及所有尸检和活检的病理学检查。 在过去的一年中，筛选了18只动物，并分配给两个SIV子项目（PI Apetrei和Orandle）。这两个项目的5年期预计动物总数为42只。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of the nonhuman primate and laboratory core is to provide highly integrated clinical management and laboratory investigations to project leaders and individual investigators. IACUC approval is made separately and individually to each subproject supported by the nonhuman primate and laboratory core. The nonhuman primate and laboratory core purchases, houses and cares for the macaques used in this project. The core is responsible for the daily clinical care of animals and experimental animal procedures such as physical examinations, inoculations with virus, blood collection, intestinal biopsies, and lymph node biopsies. The laboratory component of the core performs hematology and clinical chemistry on peripheral blood, flow cytometry for quantitation of lymphocyte cell types (CD3, CD4,CD8) and immunologic assays including quantitation of CD8 T cell responses using tetramer technology and intracellular cytokine stain. The core also performs routine bacterial cultures, SIV isolation and quantitation, and pathologic examination of all necropsies and biopsies performed on animals utilized in these studies. In the past year 18 animals were screened and allocated for two SIV subprojects (PI Apetrei and Orandle). The total number of animals anticipated for the 5 year term of the two projects is 42.