LSU VETERINARY COBRE: TNPRC NON-HUMAN PRIMATE CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 非人灵长类动物和实验室核心的目标是为项目负责人和个人研究人员提供高度集成的临床管理和实验室调查。IACUC对由非人类灵长类动物和实验室核心支持的每个子项目分别和单独进行批准。 非人灵长类动物和实验室核心购买、安置和照顾这个项目中使用的猕猴。核心负责动物的日常临床护理和实验动物程序，如体检、接种病毒、采血、肠道活检和淋巴结活检。核心的实验室部分负责外周血液的血液学和临床化学，流式细胞术定量淋巴细胞类型(CD3、CD4、CD8)和免疫学分析，包括使用四聚体技术和细胞内细胞因子染色定量CD8 T细胞反应。该中心还进行常规细菌培养、SIV分离和定量，以及对这些研究中使用的动物进行的所有身体和活组织的病理检查。 在过去的一年里，对18只动物进行了筛选，并将其分配给两个SIV子项目(Pi Apetrei和Orandle)。这两个项目在5年期间预计的动物总数为42只。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of the nonhuman primate and laboratory core is to provide highly integrated clinical management and laboratory investigations to project leaders and individual investigators. IACUC approval is made separately and individually to each subproject supported by the nonhuman primate and laboratory core. The nonhuman primate and laboratory core purchases, houses and cares for the macaques used in this project. The core is responsible for the daily clinical care of animals and experimental animal procedures such as physical examinations, inoculations with virus, blood collection, intestinal biopsies, and lymph node biopsies. The laboratory component of the core performs hematology and clinical chemistry on peripheral blood, flow cytometry for quantitation of lymphocyte cell types (CD3, CD4,CD8) and immunologic assays including quantitation of CD8 T cell responses using tetramer technology and intracellular cytokine stain. The core also performs routine bacterial cultures, SIV isolation and quantitation, and pathologic examination of all necropsies and biopsies performed on animals utilized in these studies. In the past year 18 animals were screened and allocated for two SIV subprojects (PI Apetrei and Orandle). The total number of animals anticipated for the 5 year term of the two projects is 42.