Gene Expression Profiling and Clinical Characterization of Phototoxicity

光毒性的基因表达谱和临床特征

• 批准号: 10262182
• 负责人: Heidi Kong
• 金额: $ 1.74万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10262182
• 关键词: Antibiotics; Biopsy; Clinical; Cutaneous; Cytochrome P450; Data; Data Set; Diuretics; Doxycycline; Gene Expression; Gene Expression Profiling; Genotype; Goals; Investigation; Light; Microarray Analysis; Non-Steroidal Anti-Inflammatory Agents; Pathway interactions; Pharmaceutical Preparations; Phototoxicity; Protocols documentation; Reaction; Recommendation; Role; Sampling; Serum; Skin; Stains; Sunscreening Agents; Testing; UV Radiation Exposure; Ultraviolet Rays; Visible Radiation; Voriconazole; antimicrobial; healthy volunteer; response; transcriptome sequencing; translational approach; ultraviolet

In 2010, the protocol was amended to allow us to treat a subset of returning healthy volunteers with doxycycline to obtain additional biopsies. In 2011, we completed treatment of the additional healthy volunteers. In 2012, we completed the analysis of immunohistochemical staining of the additional biopsies obtained from healthy volunteers treated with doxycycline. In fiscal year 2013, we investigated the serum levels of doxycycline from the healthy volunteers who participated in the study in previous years. In fiscal year 2017, the protocol was amended to allow additional investigation of skin samples post-UV exposure which were inconclusive. We are currently analyzing the microarray data in the context of an RNAseq dataset to determine if there are consistent findings.

2010年，该方案进行了修订，允许我们用强力霉素治疗一部分返回的健康志愿者，以获得额外的活组织检查。2011年，我们完成了对新增健康志愿者的治疗。2012年，我们完成了对接受强力霉素治疗的健康志愿者的额外活检的免疫组织化学染色分析。在2013财年，我们调查了前几年参与研究的健康志愿者的血清强力霉素水平。在2017财政年度，该协议进行了修订，允许对紫外线照射后的皮肤样本进行额外的调查，但调查结果尚无定论。我们目前正在分析RNAseq数据集背景下的微阵列数据，以确定是否有一致的发现。