Gene Expression Profiling and Clinical Characterization of Phototoxicity

光毒性的基因表达谱和临床特征

• 批准号: 7733329
• 负责人: Heidi Kong
• 金额: $ 1.17万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7733329
• 关键词: Biopsy; Clinical; Computer software; Computers; Cutaneous; Cytochrome P450; Data Analyses; Diuretics; Doxycycline; Equipment; Gene Expression; Gene Expression Profiling; Genotype; Goals; Light; Microarray Analysis; Non-Steroidal Anti-Inflammatory Agents; Pathway interactions; Pharmaceutical Preparations; Phase; Photography; Phototoxicity; Procedures; RNA; Reaction; Recommendation; Role; Skin; Sunscreening Agents; Testing; Ultraviolet Rays; Visible Radiation; Voriconazole; antimicrobial; healthy volunteer; human subject; translational approach; ultraviolet

In fiscal year 2008, we finalized phototesting of human subjects before and after doxycycline ingestiont to obtain clinical information on doxycycline-induced cutaneous phototoxicity and skin biopsies for microarray analysis. Phototesting procedures utilized 1) a solar simulator with different ultraviolet and light filters, 2) photography equipment, and 3) a colorimeter. Skin biopsies have undergone ribonucleic acid (RNA) isolation followed by hybrization on microarray chips. The project is currently in the microarray data analysis phase which has required sufficiently-powered computer equipment and software.

在2008财年，我们完成了前后对人类受试者的摄影测试 多西环素摄入以获得多西环素诱导的皮肤炎的临床信息 用于微阵列分析的光毒性和皮肤活组织检查。使用的光测试程序1）a 具有不同紫外线和滤光器的太阳模拟器，2）摄影设备，以及3） 色度计。皮肤活检进行核糖核酸（RNA）分离， 在微阵列芯片上杂交。该项目目前正在进行微阵列数据分析 这一阶段需要强大的计算机设备和软件。