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Gene Expression Profiling and Clinical Characterization of Phototoxicity

Gene Expression Profiling and Clinical Characterization of Phototoxicity
光毒性的基因表达谱和临床特征
批准号：
7733329
负责人：
Heidi Kong
金额：
$1.17万
依托单位：
DIVISION OF BASIC SCIENCES - NCI
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/7733329
关键词：
BiopsyClinicalComputer softwareComputersCutaneousCytochrome P450Data AnalysesDiureticsDoxycyclineEquipmentGene ExpressionGene Expression ProfilingGenotypeGoalsLightMicroarray AnalysisNon-Steroidal Anti-Inflammatory AgentsPathway interactionsPharmaceutical PreparationsPhasePhotographyPhototoxicityProceduresRNAReactionRecommendationRoleSkinSunscreening AgentsTestingUltraviolet RaysVisible RadiationVoriconazoleantimicrobialhealthy volunteerhuman subjecttranslational approachultraviolet

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英文摘要
In fiscal year 2008, we finalized phototesting of human subjects before and after doxycycline ingestiont to obtain clinical information on doxycycline-induced cutaneous phototoxicity and skin biopsies for microarray analysis. Phototesting procedures utilized 1) a solar simulator with different ultraviolet and light filters, 2) photography equipment, and 3) a colorimeter. Skin biopsies have undergone ribonucleic acid (RNA) isolation followed by hybrization on microarray chips. The project is currently in the microarray data analysis phase which has required sufficiently-powered computer equipment and software.
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