EFRI-BioFlex: Cellphone-based Digital Immunoassay Platform for High-throughput Sensitive and Multiplexed Detection and Distributed Spatio-Temporal Analysis of Influenza

全世界迫切需要便携式、快速、敏感和特定的流感监测系统。该倡议建议在灵活、具有成本效益的现场便携式远程医疗平台内从根本上改善流感的诊断情况。他们将开发一种数字免疫分析系统，该系统利用新颖的电自适应和可编程微流体技术，以及基于手机的多光谱荧光细胞术和计算显微镜工具。这个被提议的系统将产生由数十万个包含液滴的编码粒子组成的阵列。这些颗粒将首先使用惯性微流控分选进行均匀分割，以便每滴有一个颗粒，其中流感亚型抗原和相关酶联抗体三明治的存在将导致一个？开还是关？荧光信号在每滴。点亮的液滴数量（对应于具有不同亚型特异性抗体的特定颜色和形状编码粒子）将使用基于手机的多光谱成像细胞术系统并行成像。通过在同一部手机上运行的定制开发的智能应用程序，这些免疫分析图像将立即被分析，以灵敏和快速地检测各种流感亚型，测试结果也将无线传输到安全服务器，从而实现实时的时空映射和疾病分析。

There is an urgent need for portable, rapid, sensitive and specific influenza surveillance systems worldwide. The PI proposes to fundamentally improve the diagnostic landscape of influenza within a flexible, cost-effective, and field portable telemedicine platform. They will develop a digital immunoassay system that makes use of novel electro-adaptive and programmable microfluidics technologies as well as cellphone based multispectral fluorescent cytometry and computational microscopy tools. The proposed system will create arrays of hundreds of thousands of encoded particles containing fluid droplets. These particles will be first segmented equally using inertial-microfluidic sorting so that there is a single particle per drop, where the presence of influenza subtype antigens and the associated enzyme-linked antibody sandwich will lead to an ?on or off? fluorescence signal in each drop. The number of drops lighting up (corresponding to specific color- and shape-coded particles with different subtype-specific antibodies) will then be imaged all in parallel using a cellphone-based multispectral imaging cytometry system. Through a custom-developed smart application running on the same cellphone, these immunoassay images will immediately be analyzed for sensitive and rapid detection of various influenza subtypes, where the test results will also be transmitted wirelessly to secure servers, enabling real-time spatio-temporal mapping and analysis of the disease.