Specific elimination of anti-DNA antibody-producing cells in systemic lupus erythematosus.

特异性抗DNA抗体的产生是治疗系统性红斑狼疮(SLE)的首选方法之一。为此，我们在体外用DNA-D-谷氨酸-D-赖氨酸(DNA-D-GL)诱导DNE特异性耐受，该耐受原的加入显著减少了SLE淋巴细胞在体外自发产生的抗ds和/或抗ss DNA抗体。无反应性是DNA特异性的。我们还使用了与细胞毒剂新卡西汀(NCS)偶联的抗独特型(ID)单抗。产生抗DNA自身抗体的人B细胞克隆可被抗ID-NCS结合物体外杀伤，而表达无关特异性的ID的克隆不受影响。抗ID-NCS在体外也能特异性地抑制人类淋巴细胞产生的抗DNA自身抗体，这种方法在流产克隆方面将具有显著的优势，并将为SLE的治疗提供一种有效的治疗方法。

The specific manipulation of anti-DNA antibody production is one of the prefered treatment of systemic lupus erythematosus (SLE). For this purpose, we induced DNE-specific tolerance in vitro using DNA-Dglutamic acid-D-lysine(DNA-D-GL).The addition of the tolerogen reduced markedly the in vitro production of spontaneously occuring anti-ds and/or anti-ss DNA antibodies by SLE lymphocytes. The unresponsiveness was DNA-specific.We also used monoclonal anti-idiotypic (Id) antibodies which were conjugated with neocarzinostatin (NCS), a cytotoxic agent. Human B cell clones, which produce anti-DNA autoantibodies, were killed by by in vitro treatment with anti-Id-NCS conjugates, while clones expressing an Id with irrelevant specificity were unaffected. Anti-Id-NCS also specifically suppressed anti-DNA autoantibodyproductin in vitro by human lymphocytes.This approach will have a significant advantages in aborting clones, and will result in a potent therapeutic treatment for SLE.