课题基金 / 基金详情

HOMEOTIC TRANSCRIPTION FACTORS THAT BIND TO FOLLICLE-STIMULATING HORMONE SUBUNITE GENE

HOMEOTIC TRANSCRIPTION FACTORS THAT BIND TO FOLLICLE-STIMULATING HORMONE SUBUNITE GENE
与卵泡刺激激素亚基基因结合的同源转录因子
批准号:
12470217
负责人:
KATO Yukio
金额:
$9.09万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2000
资助国家:
日本
项目状态:
已结题
起止时间:
2000 至 2002

项目摘要

项目成果

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中文摘要
翻译
(1)利用酵母单杂交系统克隆了4个结合猪促卵泡激素亚基基因上游区域的转录因子。(2)克隆的核苷酸序列具有同源区,识别特定的DNA序列。(3)成功制备了重组转录因子,凝胶迁移实验证实了重组转录因子对FSHβ基因的特异性结合。(4)将含有克隆转录因子的表达载体与FSHβ基因5′上游融合的报告载体一起转染cho细胞,观察到重组转录因子对FSHβ基因表达有一定的刺激作用。
英文摘要
(1) FOUR TRANSCRIPTION FACTORS THAT BIND TO THE UPSTREAM REGION OF PORCINE FOLLICLE STIMULATING HORMONE SUBUNIT GENE HAVE BEEN CLONED USING YEAST ONE-HYBRID SYSTEM.(2) THE NUCEOTIDE SEQUENCES OF CLONES HAVE HOMEOTIC REGION, WHICH RECOGNIZES SPECIFIC DNA SEQUENCES.(3) PRODUCTION OF RECOMBINANT TRANSCRIPTION FACTOR HAVE BEEN SUCCEEDED AND THE GEL MOBILITY SHIFT ASSAY DEMONSTRATED SPECIFIC BINDING FOR FSH β GENE(4) EXPRESSION VECTORS CONTAINING CLONED TRANSCRIPTION FACTORS WERE TRANSFECTED IN THE CHO CELLS TOGETHER WITH A REPORTER VECTOR FUSED WITH THE 5'-UPSTREAM REGION OF THE FSHβ GENE AND SOME STIMULATION OF THE GENE EXPRESSIO WAS OBSERVED.
期刊论文(106)
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会议论文
Nishida,N.: "Critical role of nitric oxide in expression of porcine LH receptor at transcription and post-transcription levels"Experimental and Clinical Endocrinology and Diabetes. 108. 424-429 (2000)
Nishida,N.:“一氧化氮在转录和转录后水平猪 LH 受体表达中的关键作用”实验和临床内分泌学和糖尿病。
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通讯作者:
Nishida,N.: "Transcription facgtor genes, c-fos and c-jun, in developing porcine granulosa cells in vitro"Journa of Reproduction and Development. 46. 167-175 (2000)
Nishida,N.:“转录因子基因 c-fos 和 c-jun 在体外培养猪颗粒细胞中的作用”《生殖与发育杂志》。
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Iwasawa A: "Changes in thyrotropin messenger RNA and circulating hormone levels during induced molting of the hen (Gallus domesticus)"J Reprod Dev 2002;48:489-496. 48. 489-496 (2002)
Iwasawa A:“在母鸡诱导换羽过程中促甲状腺素信使 RNA 和循环激素水平的变化”J Reprod Dev 2002;48:489-496。
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通讯作者:
Nishida, N.: "Expression of endothelial nitric oxide synthase gene in cultured porcine granulosa cells after FSH stimulation"Jounal of Molecular Endocrinology. 26. 259-265 (2001)
Nishida, N.:“FSH 刺激后培养的猪颗粒细胞中内皮一氧化氮合酶基因的表达”分子内分泌学杂志。
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