The study of molecular marker expression in evaluation for the malignancy potential of colorectal cancer

我们研究了分子标志物表达在大肠癌恶性潜能评估中的作用.（1）血清学分析表明，肝癌组术前血清HGF、EGF水平明显高于对照组（P < 0.0001）。(2)免疫组化结果显示，HGF、c-Met和HGF在癌细胞中均呈强阳性表达，且在癌细胞中共定位. TGF-β（1）免疫组化染色：淋巴结转移组阳性率高，向血管系统浸润组阳性率高。(2)血清学检查发现，肿瘤患者与非肿瘤患者之间TGF-β1水平存在显著性差异，且随着肝转移和静脉浸润的进展，TGF-β1水平逐渐升高.层粘连蛋白（1）结肠癌患者血清层粘连蛋白水平为606.3± 260.2ng/ml，明显高于正常对照组的258.0± 92.0ng/ml（P<0.0001）。(2)层粘连蛋白的阳性率为89.3%，高于CEA的38.0%和CA 19 -9的19.5%。(3)肝转移受试者的平均血清层粘连蛋白为668.0±274.7 ng/ml，显著高于无肝转移受试者的585.2±252.5 ng/ml（p=0.0472）。(4)高水平层粘连蛋白组（大于或等于520 ng/ml）的生存率显著低于低水平层粘连蛋白组（<350 ng/ml）（P=0.0451）。采用考克斯的比例风险回归模型进行单因素和多因素分析，结果显示血清层粘连蛋白是结直肠癌的独立预后因素，沿着肝、肺和腹膜转移。

We investigated the molecular marker expression in evaluation for the maliginancy potential of colorectal cancer.1. HGF(hepatocyte growth factor), EGF(epidermal growth factor)(1) Serological analysis demonstrated that preoperative serum HGF and EGF level was significantly higher in the cancer group than in the control group (p < 0.0001).(2) Immunohistochemical analysis demonstrated the strong expression and colocalization of HGF, c-Met and HGF in the cancer cells.2. TGF-β(1) In immunohistological staining, the positive rate became higher in lymph-node metastases and in progressing invasion into the vascular system.(2) In serological examination, a significant difference was found between cancer cases and non-cancer cases, and TGF-β1 level rose with progressing liver metastasis and venous invasion.3. Laminin(1) Mean serum laminin in subjects with colon cancer was 606.3±260.2 ng/ml, significantly higher than that of 258.0±92.0 ng/ml in normal controls (p<0.0001).(2) The positive rate was higher at 89.3% for laminin vs 38.0% for CEA and 19.5% for CA19-9.(3) Mean serum laminin in subjects with hepatic metastases was 668.0±274.7 ng/ml, significantly higher than that of 585.2±252.5 ng/ml in subjects without hepatic metastases (p=0.0472).(4) Survival rates were significantly lower in the high (【greater than or equal】520 ng/ml) than in the low laminin group (<350 ng/ml) (P=0.0451). Univariate and multivariate analysis, using Cox' s proportional hazard regression model, showed serum laminin is an independent prognostic factor in colorectal cancer, along with hepatic, pulmonary and peritoneal metastases.