Pathogenesis on reactivation of viral infections in immunocompromised hosts and its prophylaxis and therapy.

移植医学的最新进展导致免疫受损宿主的增加。疱疹病毒在免疫缺陷中的再活化作用日益重要，为了阐明这些病毒再活化的机制并建立预防和治疗，我们建立了定量病毒载量和针对疱疹病毒的病毒特异性细胞免疫。使用这些系统，我们发现了以下发现。1.实时荧光定量PCR检测EB病毒载量有助于造血干细胞移植患者淋巴组织增生性疾病的诊断。此外，定量检测病毒特异性T淋巴细胞对确定治疗方案也有帮助.病毒型别与新生儿单纯疱疹病毒感染的预后及复发有关.巨细胞病毒通过母乳传播在早产儿中很重要，但症状性感染在人群中很少见。

Recent advances in transplantation medicine result in an increase of immunocompromised hosts. The importance of herpes viruses, which reactivate in immunodeficiency, is increasing.To clarify the mechanism on reactivation of these viruses and to establish prophylaxis and treatment, we established quantifying viral load and viral specific cellular immunity against herpesviruses. Using these systems, we found the following findings.1. Quantitation of Epstein-Barr viral load using real-time PCR was useful for the diagnosis of lymphoproliferative disorders in patients with hematopoietic stem cell transplantation. Furthermore, quantifying viral specific T lymphocytes was useful for determing the treatment.2. Viral type was related with the prognosis and relapse of neonatal herpes simplex virus infections.3. Transmission of cytomegalovirus via breast milk was important among premature infants, but symptomatic infections were rare in the population.