Basic and clinical research of viral gastroenteritis in children and its risk evaluation

我们生产了诺如病毒和萨波病毒的新病毒样颗粒（vlp），以更新病毒遗传变异的后果。免疫层析（IC）诊断试剂盒是由这些新的VLPs开发/改进的。我们除了对轮状病毒、诺如病毒、萨波病毒、腺病毒和星状病毒进行了分子流行病学研究外，还在腹泻粪便样本中发现了新型腹泻病毒，如爱知病毒、parechovirus、bocavvirus和藏红花病毒。用鼠诺如病毒作为新的替代物来评价人诺如病毒的消毒剂、加热和抗病毒活性。我们挑战诺如病毒的多细胞培养试验，但目前尚未成功。我们还检测了诺如病毒和萨波病毒与唾液和肠细胞的组织血型抗原的结合活性。有趣的是，诺如病毒表现出较高的结合活性，而萨波病毒则没有。

We have produced new virus-like particles(VLPs) of norovirus and sapovirus in order to update the consequences of viral genetic variability. The immunochromatography(IC) diagnosis test kits have been developed/improved by these new VLPs. In addition to the molecular epidemiological study of rotavirus, norovirus, sapovirus, adenovirus and astrovirus that we have done, the novel diarrheal viruses such as Aichi virus, parechovirus, bocavirus and Saffold virus have also been found in diarrheal stool samples. Murine norovirus was used as a novel surrogate to evaluate disinfectants, heating, and antiviral activity for human norovirus. We challenged multiple-cell cultivation assays for norovirus but did not succeed at this moment. We also examined the binding activities of norovirus and sapovirus to histo-blood group antigens of saliva and intestinal cells. It was interesting to find that norovirus, but not sapovirus, showed high binding activity.