Regulating cell function by introduction of the proteins enzymatically modified with synthetic functional molecules into cell membrane or cytoplasm

我们开发了一种技术，通过用合成的功能分子对目标蛋白的C末端进行酶特异性修饰，将目标蛋白转移到细胞膜和细胞质中。利用目前的技术，可以在细胞膜上用聚乙二醇脂对、靶蛋白进行修饰。这种方法通过在癌细胞膜上引入抗体，成功地提高了树突状细胞吞噬癌细胞的效率。此外，、我们还开发了一种方法，通过将带有染料、的穿透性多肽连接到目的蛋白上，并在细胞摄取修饰后的蛋白质后进行光照，目的蛋白在光照射下产生自由基、，从而将目的蛋白转移到细胞质。

We developed technologies that can transfer target proteins into cellular membrane and cytoplasm by enzymatically-specific modification of the C-terminal of target proteins with synthetic functional molecules. By the present technologies、target proteins could be modified with PEG-lipid on cellular membrane. This method realized that the efficiency in the phagocytosis of dendritic cells for cancer cells was successfully enhanced by introducing antibodies onto cancer membranes. In addition、we developed a method that can easily transfer target proteins to cytoplasm by attaching cell-penetrating peptide bearing the dye、which generates radical under photo-irradiation、to target protein and irradiating light after cellular uptake of the present modified protein.