ELECTROSTATIC PROPERTIES OF MEMBRANE

细胞膜的表面电位是细胞膜的一个重要决定因素， 细胞的生理功能。我们调查了一些 影响膜表面电位的因素。我们还 修正了用于计算表面电势的理论， Gouy-Chapman理论来解释这些因素。 质膜中的三磷酸肌醇脂质可能含有多达五个 负电荷。我们之前研究了钙， 镁、钾、质子和其它阳离子与三磷酸肌醇， 并测定了结合到脂质的阳离子的数量， 生理相关的条件。我们还研究了质子滴定 曲线的脂质，并发现有一个合作的相互作用 在肌醇头基的两个单酯基之间。我们提出 这种相互作用可能会对网络产生重要影响， 在生理条件下三磷酸肌醇脂质上的电荷。

The surface potential of cellular membranes is an important determinant in the physiological function of the cell. We have investigated a number of factors that affect the surface potential of membranes. We have also modified the theory that has been used to calculate the surface potential, the Gouy-Chapman theory, to account for these factors. Triphosphoinositide lipids in plasma membranes could have up to five negative charges. We previously investigated the interaction of calcium, magnesium, potassium, protons and other cations with triphosphoinositides, and determined the number of cations bound to the lipid under physiologically-relevant conditions. We also investigated proton titration curves of the lipid and found that there was a cooperative interaction between the two monoester groups in the inositol headgroup. We proposed that this interaction could have important consequences in terms of the net charge on the triphosphoinositide lipid under physiological conditions.