THEORETICAL STUDIES ON PH-ZONE REFINING COUNTERCURRENT CHROMATOGRAPHY

用一组DNP-氨基进行了一系列的基本实验 酸和甲基叔丁基组成的两相溶剂体系 乙醚/水。将保留酸(TFA)加入固定相 以及不同浓度的洗脱剂碱(NH3)至流动相。 结果与数学分析相一致。 在目前系统中运行的流体动力机构的基础上开发的。 研究的总体结果表明，目前的方法符合 在许多方面与置换层析非常相似：一 溶质抑制其他物质的电离或亲和力，从而改变 后者的分配系数；在列中有一列急剧的 形成定义的溶质带，并且每个带以相同的速度一起移动 杂质集中在每个溶质带的边界上； 然而，在按键的动作上有截然不同的不同 试剂。在pH区精制CCC中，保留酸转移溶质 从流动相到前端的固定相的分子 溶质带的位置，而置换层析中的置换剂 置换吸附在后方固定相上的溶质分子 溶质带的末端。这些相互逆转的按键动作 溶质带两端的试剂产生相同的 洗脱图谱。从这个观点来看，本技术还可以 被称为“反向驱替CCC”。这一术语进一步暗示了 位移CCC的存在以及反向位移的存在 层析法。

A series of basic experiments has been performed with a set of DNP-amino acids and two-phase solvent system composed of methyl tertiary-butyl ether/water. The retainer acid (TFA) was added to the stationary phase and the eluent base (NH3) to the mobil phase at various concentrations. The results were found to be consistent with the mathematical analyses developed on the hydrodynamic mechanism operating in the present system. The overall results of the studies revealed that the present method bears a close resemblance to displacement chromatography in many aspects: One solute suppresses the ionization or affinity of others to alter the partition coefficient of the latter; in the column a train of sharply defined solute bands is formed and each band moves together at the same rate; impurities are concentrated at the boundaries of each solute bands; etc. However, there is a contrasting difference in the action of the key reagents. In pH-zone-refining CCC, the retainer acid transfers solute molecules from the mobile phase to the stationary phase at the front end of the solute bands whereas in displacement chromatography the displacer displaces solute molecules absorbed onto the stationary phase at the rear end of the solute bands. These mutually reversed actions of the key reagents at the opposite ends of the solute bands produce identical elution profiles. From this viewpoint, the present technique may also be called "Reverse Displacement CCC". This term further suggests the presence of displacement CCC as well as that of reverse displacement chromatography.