INFECTION OF NON-HEMATOPOIETIC CELLS WITH HIV-1 AND HIV-2

已知HIV-1感染多种非淋巴细胞系，其中 进入的方式被假定为涉及额外的或 独立受体或第二信使样分子。 的程度 病毒在这些细胞系中的复制有很大的不同， 生产到流产感染提示宿主依赖性控制 病毒复制 这些模型系统是识别 参与病毒复制的细胞和病毒因子。 为了 我们选择的靶细胞是SKNSH、SKNMC （神经母细胞瘤细胞系）、HTB-14（来源于星形细胞瘤）、A-204和 RD 114（横纹肌肉瘤）、A1 N4、正常乳腺细胞系、MCF-7、BT-20和BT 483乳腺癌细胞株，TE 85和SAOS-2，2成骨肉瘤 细胞系和人胚胎细胞系L132。 其中一些细胞系 同时感染了HIV-1和HIV-2。 病毒的动力学 复制（通过病毒整合和未整合的合成来评估） DNA、RNA和蛋白质）在这些细胞系中进行分析。 两种病毒 和细胞因子，如激酶、DNA结合蛋白和细胞因子， 这可能决定了病毒复制的不同程度， 这些不同的细胞系将在RNA和蛋白质 水平

HIV-1 has been known to infect a variety of non-lymphoid cell lines, where the mode of entry has been postulated to involve an additional or an independent receptor or a second messenger-like molecule. The extent of viral replication in these cell lines is vastly different ranging from productive to abortive infections suggestive of host dependant controls on viral replication. These model systems are ideal for identification of cellular and viral factors involved in viral replication. In order to investigate this the target cell we have chosen to work are SKNSH, SKNMC (neuroblastoma cell lines), HTB-14 (derived from an astrocytoma), A-204 and RD114 (rhabdomyosarcoma), A1N4, a normal breast line, MCF-7, BT-20 and BT 483 mammary carcinoma cell lines, TE85 and SAOS-2, 2 osteogenic sarcoma cell lines and L132, a human embryonic cell line. Some of these cell lines have been infected with both HIV-1 and HIV-2. The kinetics of viral replication (assessed by synthesis of viral integrated and unintegrated DNA, RNA and proteins) is being analyzed in these cell lines. Both viral and cellular factors, such as kinases, DNA binding proteins and cytokines, that may govern the different extents to which viral replication occurs in these different cell lines will be investigated at the RNA and protein level.