ASSESSMENT OF HIV INFECTION IN SPANISH SERONEGATIVE IVDAS AND THEIR PARTNERS

静脉注射药物滥用者是艾滋病毒传播的主要目标 in Spain. 艾滋病毒抗体的流行率为8-15%， IVDA，尽管由于大量的 这些人中的一部分可能是潜伏感染。 的存在 用PCR方法检测HIV潜伏感染情况。 制备基因组DNA 来自32名IVDA血清阴性伴侣和5名 血清阳性区域。 引物来源于gag（SK 38/39）和env (68/69）区域。 还对所有样本进行了HIV抗原血症检测。 11/32 IVDAs的异性伴侣和5名血清阳性性乱伴侣 科目 32名IVDA异性伴侣中有11名为gag阳性 PCR测序。 3例env序列阳性。 所有5异性恋 性滥交感染者的伴侣呈阳性，但4例也有 HIV抗体。 13例PCR检测呈阳性但未检测出 HIV抗体HIV抗原阴性。 我们的研究表明 在西班牙IVDA中可能存在高比例的隐性HIV感染。 我们目前正在评估IVDA在更大人群中的患病率 以及血清反应阴性的男同性恋者及其伴侣。

Intravenous drug abusers (IVDAs) are a major target for the spread of HIV in Spain. A prevalence rate of 8-15% of HIV antibodies among partners of IVDAs, although higher rates have been suggested since a substantial proportion of these people may be latently infected. The presence of latent HIV infection was investigated by PCR. Genomic DNA was prepared from (PBMCs) of 32 seronegative partners of IVDAs and 5 partners of seropositive regions. Primers were derived from the gag (SK38/39) and env (68/69) regions. All samples were also tested for HIV antigenaemia. 11/32 heterosexual partners of IVDAs and 5 partners of seropositive promiscuous subjects. 11 of 32 heterosexual partners of IVDAs as positive for gag sequences by PCR. 3 were positive for env sequences. All 5 heterosexual partners of the promiscuous infected subjects were positive, but 4 also had HIV antibodies. The 13 subjects who were positive by PCR but did not have antibodies to HIV were negative for HIV antigen. Our study suggests that there may be a high proportion of silent HIV infection in Spanish IVDAs. We are at present evaluating the prevalence in larger population of IVDAs and homosexual men and their partners who remain seronegative.