INFECTION OF NON-HEMATOPOIETIC CELLS WITH HIV-1 AND HIV-2

已知HIV-1感染多种非淋巴细胞系，其中 进入的方式被假定为涉及额外的或 独立受体或第二信使样分子。 为了 为了研究病毒在这些细胞中复制的机制， 测试了这些细胞系与HIV-IIIB株的感染性， HIV-2 ROD毒株。 我们检测的细胞系是SKNSH、SKNMC （神经母细胞瘤细胞系）、HIB-14（来源于星形细胞瘤）、A-204和 RD114（横纹肌肉瘤）。 所有这些人都感染了HIV-1， HIV-2. 我们正在研究这两种病毒的复制动力学， 这些细胞系，基因表达模式（即，相比 在淋巴细胞中观察到的。 研究也在进行中， 其它可能靶细胞如乳腺上皮细胞的感染性 这些细胞可能与母体的病毒传播有关， 胎儿路线 这些研究也将使我们能够更好地定义细胞 或允许感染过程的组织特异性因素。

HIV-1 has been known to infect a variety of non-lymphoid cell lines, where the mode of entry has been postulated to involve an additional or an independent receptor or a second messenger-like molecule. In order to investigate the mechanism of viral replication in these cells we first tested the infectability of these cell lines with the HIV-IIIB strain and HIV-2 ROD strain. The cell lines we tested were SKNSH, SKNMC (neuroblastoma cell lines), HIB-14 (derived from an astrocytoma), A-204 and RD114 (rhabdomyosarcoma). All of them were infectable with both HIV-1 and HIV-2. We are studying the kinetics of replication of the 2 viruses in these cell lines, the pattern of gene expression (i.e., comparison with that observed in lymphoid cells. Studies are also in progress to examine the infectability of other possible target cells such as mammary epithelial cells which may have implications for virus transmission by the maternal- fetal route. These studies would also enable us to better define the cell or tissue specific factors that permit the course of the infection.