IMMUNOREGULATION AND IMMUNE RECOGNITION IN FILARIASIS AND NON-FILARIAL DISEASES

这个项目的目的是勾勒出 参与调节体液和细胞反应 丝虫病和其他疾病的患者状态并确定 那些负责诱导这些反应的抗原。 免疫调节研究已经研究了这种现象 微丝虫病患者中抗原特异性无能的研究 在抗体(B细胞)水平上证明这种无能 淋巴因子(T细胞)反应性。此外，类似的T细胞无能 在活动性盘尾丝虫病患者中被发现。这个 遗传因素和抗原组成对此的影响 无能已经开始使用分子生物学工具，并定义了 抗原。 寄生虫抗原驱动抗体产生的体外模型 以及寄生虫特异性和HTLV-I转化的T细胞克隆 已经被开发成更详细地理解那些 调节抗体产生的机制(特别是免疫球蛋白和 在丝虫病和非丝虫病中)。 血吸虫病患者丝虫特异性IgE和IgG的定性检测， 淋巴丝虫病和盘尾丝虫病表明 抗原识别在不同的患者群体中有所不同 丝虫病的不同临床表现。使用这些 技术，由患者唯一识别的抗原 已经发现了血吸虫病或盘尾丝虫病。丝虫物种- 人源单抗识别的特异性抗原 来自EBV转化患者的B细胞也被 确定和表征的。此外，FPLC分馏和 丝虫抗原性成分的定性分析 寄生虫已经鉴定出两组蛋白质，它们似乎可以 从细胞和体液两方面调节免疫反应 水平。

The purpose of this project is to delineate the mechanisms involved in regulating the humoral and cellular responses in patients with filariasis and other disease states and to determine those antigens responsible for inducing these responses. Immunoregulatory studies have examined the phenomenon of antigen-specific anergy in microfilaremic patients by demonstrating this anergy at the level of antibody (B cell) and lymphokine (T cell) responsiveness. Further, similar T cell anergy has been found among patients with active onchocerciasis. The influence of genetic factors and antigen composition on this anergy has begun using molecular biologi-cal tools and defined antigens. In vitro models of parasite-antigen driven antibody production as well as parasite-specific and HTLV-I transformed T cell clones have been developed to understand in more detail those mechanisms regulating antibody production (particularly IgG and IgE) in filarial and non-filarial diseases. Qualitative analysis of filaria-specific IgE and IgG in loiasis, lymphatic filariasis, and onchocerciasis have indicated patterns of antigen recognition which differ among groups of patients with different clinical manifestations of filariasis. Using these techniques, antigens recognized exclusively by patients with loiasis or onchocerciasis have been identified. Filarial species- specific antigens recognized by human monoclonal antibodies derived from EBV-transformed patient B cells have also been identified and characterized. Further, FPLC fractionation and qualitative analysis of the antigenic components of filarial parasites has identified two sets of proteins which appear to regulate the immune response at both the cellular and humoral level.