PHYSICAL CHEMISTRY OF BIOLOGICAL MACROMOLECULES

这个项目的目的是研究一个广泛的物理性质 各种生物大分子，目标是将这些 大分子的结构和功能的性质。 的 重点是这些相互作用的热力学 大分子及其分子大小和形状。 分析 超浓缩和数学建模是主要的研究内容 使用的技术。 对肌动蛋白的研究一直致力于研究 肌动蛋白与肌动结合素。 关于白细胞介素2受体的研究已经 涉及建立单体分子量，并确定 无论它是否具有聚合特性。 研究人类 血小板糖蛋白GPIIb/IIIa已被用于研究其 与纤维蛋白原的相互作用。 各种脱氧核苷酸的研究已经 直接研究可逆的热力学 作为结构函数的单体-二聚体平衡。 艾滋病毒研究 逆转录酶已经被用于研究 P66亚基和P66亚基的均匀缔合的热力学 P66与P51亚基的异质缔合。 研究 chromagranin A已经被用于研究pH的影响， 和钙离子浓度对聚集性能的影响 分子。

The purpose of this project is to study the physical properties of a wide variety of biological macromolecules, with the goal of correlating these properties to the structure and function of the macromolecules. The emphasis is on the thermodynamics of the interactions of these macromolecules and on their molecular size and shape. Analytical ultracentrifugation and mathematical modeling are the principal research techniques used. Studies on actin have been directed toward investigating the interaction of actin with actobindin. Studies on the interleukin2 receptor have been directed to establishing the monomer molecular weight, and determining whether or not it has aggregative properties. Studies on the human platelet glycoprotein GPIIb/IIIa have been directed toward studying its interaction with fibrinogen. Studies of various deoxynucleotides have been directed toward investigating the thermodynamics of the reversible monomer-dimer equilibrium as a function of structure,. Studies on HIV reverse transcriptase have been directed toward investigating the thermodynamics of the homogeneous association of the P66 subunit and the heterogeneous association of P66 with the P51 subunit. Studies on chromagranin A have been directed toward investigating the effects of pH and calcium ion concentration on the aggregative properties of the molecule.