Bioanalytical Separation Using Chiral Polymers

描述(申请人提供)本项目继续致力于合成和开发用于手性分离的新型手性试剂 外消旋混合物的拆分方法。四个不同的领域 建议对这项续签提案进行调查。第一个涉及的领域 手性聚合物用作流动相添加剂的继续研究 毛细管电泳手性分离的实现 这种方法被称为胶束电动势色谱(MEKC)。焦点 继续使用聚合物表面活性剂，这种表面活性剂是通过光诱导产生的 关注继续手性的聚合物表面活性剂的性质 识别，包括1)表面活性剂尾长，2)头群变化1 3) 囊泡聚合物作为手性试剂，以及4)其他参数，如 反离子、pH和离子强度。新型手性聚合物(例如手性 间苯二酚芳烃也将在我们这一阶段的研究中得到检验。第二 这项拟议研究的一部分涉及使用聚合物表面活性剂作为 用于手性分离的固定相。有人提出，这些手性物质 聚合物可以很容易地连接到二氧化硅颗粒上，用作固定相 为了实现分离，将这些聚合物连接到二氧化硅的可能方法 以创建固定相的概述。将这种方法应用于 毛细管电色谱(CEC)为手性分离开辟了新的途径。 这项研究的第三部分涉及两者的结合 上述方法，即使用手性胶束聚合物在 手性多聚表面活性剂作为固定相的流动相 阶段。有理由期待这种方法会产生协同效应。 在大多数分居中，这一点都没有影响。我们的第四个组成部分 研究涉及到各种方法，这些方法是由 我们的研究引起了其他科学家的关注和兴趣， 主要是有机化学家。因此，这些研究在很大程度上将是合作的 在自然界中。这些研究将包括对其他类型的手性药物的检查。 聚合物作为可能的分离剂，主要在CE和CEC中。

DESCRIPTION (provided by applicant) This project continues to focus on synthesis and development of new chiral reagents foruse in chiral separation methods for resolution of racemic mixtures. Four different areas of investigations are suggested for this renewal proposal. The first area involved continued studies on the use of chiral polymers as mobile phase additives to achieve chiral separations in Capillary Electrophoresis (CE) through an approach known as micellar electrokinetc chromatography (MEKC). The focus continues to be on polymeric surfactants, which are produced by photo-induced focus on properties of polymeric surfactants which continue to chiral recognition, including 1) surfactant tail length, 2) head group variations1 3) vesicle polymers as chiral reagents, and 4) other parameters such as change in counterions, pH, and ionic strength. New chiral polymers (e.g. chiral resorcinarenes will also be examined in this phase of our research. The second part of this proposed research involves the use of polymeric surfactants as stationary phases for chiral separations. It is proposed that these chiral polymers can be easily linked to silica particles and used as stationary phases to achieve separation Possible approaches to linking these polymers to silica to crea stationary phases are outlined. The application of this approach to capillary electro chromatography (CEC) opens new avenues for chiral separation. The third component of this research involves a combination of the two approaches that are proposed above, i.e. a use of chiral micelle polymers in the mobile phase in combination with chiral polumeric surfactants as stationary phases. It is reasonable to expect that this approach will produce synergistic affects in most separations, it not at all. The fourth component of our research involves a variety of approaches which have resulted from the attention and interest that our research has engendered from other scientists, primarily organic chemists. Thus, these studies will largely be collaborative in nature. These studies will involve an examination of other kinds of chiral polymers as possible separation agents, primarily in CE and CEC.