GLYCOSYL LINKAGE ANALYSIS OF WATER SOLUBLE & INSOLUBLE LINKED GLUCANS

样品被全甲基化，并用MALDI-MS使用 2，5-二羟基苯甲酸基质。样本的甲基化程度是 使用氢氧化钠/亚甲基碘化物完成。进行MALDI-TOF-MS HP LDI 1700XP质谱仪工作在正离子模式 在30千伏、压力6×10~(-7)托的条件下。质谱仪 用葡萄糖低聚物的混合物进行校准(程度 聚合在3到20之间)。样品的水溶液 用含100 mm的50%乙腈水溶液稀释13 2，5-二羟基苯甲酸和一部分(0.5(L))应用于 质谱仪的探头尖端。样品是从 具有脉冲宽度为3的氮气激光器(=337 nm)的探头 Ns，并提供大约16(J)的能量/激光脉冲。

The sample was permethylated and analyzed by MALDI-MS using a 2,5-dihydroxybenzoic acid matrix. Methylation of the sample was accomplished using NaOH/methyliodide. MALDI-TOF-MS was performed with an HP LDI 1700XP mass spectrometer operated in the positive ion mode at 30 kV and with a pressure of 6 x 10-7 Torr. The mass spectrometer was calibrated with a mixture of glucose oligomers (degree of polymerization between 3 and 20). Aqueous solutions of samples were diluted 13 with aqueous 50% acetonitrile containing 100 mM 2,5-dihydroxybenzoic acid, and a portion (0.5 (L) was applied to the probe tip of the mass spectrometer. Samples were desorbed from the probe tip with a nitrogen laser ((=337 nm) having a pulse width of 3 ns and delivering approximately 16 (J of energy/laser pulse.