Bioanalytical Separation Using Chiral Polymers

描述（由申请人提供）本项目继续关注 新型手性分离试剂合成与开发 外消旋混合物的拆分方法。四个不同的领域 建议对这项更新建议进行调查。第一个涉及的领域 继续研究使用手性聚合物作为移动的相添加剂， 在毛细管电泳（CE）中实现手性分离， 这种方法被称为胶束电动势色谱法（MEKC）。重点 继续是聚合物表面活性剂，这是由光诱导产生的 专注于聚合物表面活性剂的性质， 识别，包括1）表面活性剂尾长，2）头基变化1 3） 作为手性试剂的囊泡聚合物，和4）其它参数，例如 平衡离子、pH和离子强度。新的手性聚合物（例如手性聚合物） 间苯二酚芳烃也将在我们的研究的这一阶段进行研究。第二 所提出的研究的一部分涉及使用聚合物表面活性剂， 用于手性分离的固定相。有人提出，这些手性 聚合物可以容易地连接到二氧化硅颗粒上并用作固定相 将这些聚合物与二氧化硅连接的可能方法 建立固定相。将这种方法应用于 毛细管电色谱（CEC）为手性分离开辟了新的途径。 本研究的第三个组成部分涉及两者的结合 以上提出的方法，即使用手性胶束聚合物， 移动的相与作为固定相的手性聚合物表面活性剂组合 阶段。有理由期待这种方法将产生协同效应， 在大多数分离中，它根本不起作用。我们的第四个组成部分 研究涉及各种各样的方法，这些方法来自于 我们的研究引起了其他科学家的关注和兴趣， 主要是有机化学家。因此，这些研究将在很大程度上是合作的 在自然界中。这些研究将涉及对其他类型的手性化合物的研究。 聚合物作为可能的分离剂，主要用于CE和CEC。

DESCRIPTION (provided by applicant) This project continues to focus on synthesis and development of new chiral reagents foruse in chiral separation methods for resolution of racemic mixtures. Four different areas of investigations are suggested for this renewal proposal. The first area involved continued studies on the use of chiral polymers as mobile phase additives to achieve chiral separations in Capillary Electrophoresis (CE) through an approach known as micellar electrokinetc chromatography (MEKC). The focus continues to be on polymeric surfactants, which are produced by photo-induced focus on properties of polymeric surfactants which continue to chiral recognition, including 1) surfactant tail length, 2) head group variations1 3) vesicle polymers as chiral reagents, and 4) other parameters such as change in counterions, pH, and ionic strength. New chiral polymers (e.g. chiral resorcinarenes will also be examined in this phase of our research. The second part of this proposed research involves the use of polymeric surfactants as stationary phases for chiral separations. It is proposed that these chiral polymers can be easily linked to silica particles and used as stationary phases to achieve separation Possible approaches to linking these polymers to silica to crea stationary phases are outlined. The application of this approach to capillary electro chromatography (CEC) opens new avenues for chiral separation. The third component of this research involves a combination of the two approaches that are proposed above, i.e. a use of chiral micelle polymers in the mobile phase in combination with chiral polumeric surfactants as stationary phases. It is reasonable to expect that this approach will produce synergistic affects in most separations, it not at all. The fourth component of our research involves a variety of approaches which have resulted from the attention and interest that our research has engendered from other scientists, primarily organic chemists. Thus, these studies will largely be collaborative in nature. These studies will involve an examination of other kinds of chiral polymers as possible separation agents, primarily in CE and CEC.