GENETIC STUDIES OF ROTAVIRUS PATHOGENESIS AND DEVELOPMENT OF ROTAVIRUS VACCINES

轮状病毒是引起严重腹泻的主要已知病原体。 世界各地的婴幼儿和儿童发展的需要 针对轮状病毒疾病的疫苗已经得到了广泛的认可。这个 完整的轮状病毒颗粒包含三个主要结构成分， 包裹了11个双链RNA片段的病毒基因组。 这个项目的目标有两个：(I)识别病毒基因 在轮状病毒的毒力中起主要作用的是通过使用 缺初乳灵知生菌新生猪的半同源系统 和猪轮状病毒SB-1a株的各种重组体(引起 人轮状病毒DS-1株(该病毒株 不会在诺维菌素中引起生产性感染或腹泻 仔猪)，以及(Ii)将这些信息应用于制定 发展安全有效的轮状病毒疫苗的策略 具有最佳疗效的药物。此外，这种方法可能会扩大我们的 对轮状病毒组织嗜性及控制机制的认识 在胃肠道中复制。

Rotaviruses are the major known etiologic agents of severe diarrhea in infants young children worldwide, and the need for the development of vaccines against rotavirus diseases has been widely acknowledged. The complete rotavirus particle contains three major structural components, enclosing the viral genome of eleven segments of double-stranded RNA. The goals of this project are two-fold: (i) to identify the viral genes that play a major role in virulence of rotaviruses by using a semi-homologous system of colostrum-deprived gnotobiotic newborn pigs and various reassortants of porcine rotavirus SB-1A strain (which causes diarrhea in gnotobiotic piglets) and human rotavirus DS-1 strain (which does not induce productive infection or diarrhea in gnotobiotic piglets), and (ii) to apply such information to the formulation of a strategy for the development of safe and effective rotavirus vaccines that are of optimal efficacy. In addition, this approach may expand our understanding of the mechanisms controlling rotavirus tissue tropism and replication in the gastrointestinal tract.