INVESTIGATION OF STEM CELL METABOLISM BY NMR

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 利用多能细胞形成特定的细胞类型需要了解基本代谢和影响分化的因素。在这项研究中，干细胞在13C同位素标记的底物(如葡萄糖)中生长，代谢产物通过核磁共振进行鉴定。处于不同生长阶段和不同条件下的细胞正在接受检查。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The utilization of pluripotent cells to form specific cell types requires an understanding of basic metabolism and the factors that affect differentiation. In this study, stem cells are being grown with 13Cisotopically labeled substrates such as glucose, and the products of the metabolism are identified by NMR. Cells at different stages of growth and under different conditions are being examined.