Imaging Core

核心C：抽象 节目的主旋律是剖析急性呼吸综合征中的一个新概念 窘迫综合征(ARDS)，免疫抑制是继发于 调节上皮细胞和髓系细胞活性的独特的组合途径和先天的 免疫功能。我们将肺炎作为研究的概念框架。 免疫抑制，因为肺炎是ARDS的常见原因和危险因素。教职员工 在成像核心内，一直与 这项计划在免疫生物学、脂质生物学的各个方面已经有23年的历史了 和呼吸道/肺部生物学，形成了37篇合作论文。 成像核心的主要作用将是；仔细分析细胞和分子 与细胞凋亡、坏死、铁性下垂和其他细胞死亡机制相关的过程 组织内细胞类型的需求、特征和量化、蛋白质检测 细胞死亡过程中的表达，体外细胞-细胞相互作用的检测，定量分析 体内和体外单个细胞在组织内的迁移，以及数量 趋化因子和细胞型标记物表达水平和模式的测定 各种样品在所有级别的分辨率。这些研究将采用全系列的电流 光和潜在的电子显微镜方法，包括：单色和多色 荧光显微镜、激光共聚焦显微镜、活细胞成像、透射电子 显微镜和计算机辅助形态测量分析。生物成像中心 该核心服务将被执行的目的是为了提供 向用户展示艺术微观技术。它被装备成执行连续的光学 方法包括本计划项目所必需的所有类型的光学显微镜和电子显微镜。

Core C: Abstract The overarching theme of program is to dissect a new concept that in Acute Respiratory Distress Syndrome (ARDS), immunosuppression is a signature manifestation secondary to unique, combinatorial pathways that modulate epithelial and myeloid cell viability and innate immune function. We focus on pneumonia as a conceptual framework to study immunosuppression, as pneumonia is a common cause and risk factor for ARDS. The faculty within the Imaging Core have been collaborating continuously and productively with the PI’s of this program for 23 years in various aspects of immunobiology, lipid biology andairway/pulmonary biology resulting in 37 collaborative papers. The principal roles of the imaging core will be; careful analysis of cellular and molecular processes associated with apoptosis, necrosis, ferroptosis and other cell death mechanisms as needed, characterization and quantification of cell types within tissues, examination of protein expression during cell death, examination of cell-cell interactions in vitro, quantitative analysis of cellular migration in vivo and ex vivo individual cells within tissues, and quantitative measurement of the levels and patterns of expression of chemokines and cell-type markers various samples at all levels of resolution. These studies will employ the full array of current light, and potentially, electron microscopic methods including: single and multicolor fluorescence microscopy, laser confocal microscopy live cell imaging, transmission electron microscopy and computer-aided morphometric analyses . The Center for Biologic Imaging, in which this core service will be performed, is designed for the purpose of providing state of the art microscopic technologies to its users. It is equipped to perform a continuum of optical methods including all types of light and electron microscopy essential to this Program Project.