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Characterizing molecular interactomes using TurboID

Characterizing molecular interactomes using TurboID
使用 TurboID 表征分子相互作用组
批准号：
10916059
负责人：
Mark Henderson
金额：
$12.58万
依托单位：
NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/10916059
关键词：
Automobile DrivingBasic ScienceBehaviorBiotinBiotinylationDasatinibExtramural ActivitiesHealthHumanHuman GenomeLabelLigaseMass Spectrum AnalysisMethodsMethotrexateMolecularNational Center for Advancing Translational SciencesProteinsRare DiseasesResearchSystemTranslationsdrug candidateimprovedpharmacologicprogramssmall moleculetool

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中文摘要
翻译
在此期间，合作团队开发了一些工具来探索TurboID作为接近标记工具的新用途，以定义小分子相互作用组。探索了多种TurboID结构和表达策略来表征系统的行为。一些概念验证的小分子，具有已知的作用机制，包括达沙替尼和甲氨蝶呤已被用于这些研究。该团队还寻求了一种分离生物素化蛋白的优化方法，用于随后的质谱分析和目标识别。
英文摘要
During this period, the collaborative team has developed tools to explore new uses of TurboID as a proximity labeling tool to define small molecule interactomes. A variety of TurboID constructs and expression strategies were explored to characterize the behavior of the system. Several proof-of-concept small molecules, with known mechanisms of action, including dasatinib and methotrexate have been employed for these studies. The team has also pursued an optimized method for isolating biotinylated proteins for subsequent mass spectrometry and target identification.
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