Mount Sinai HHEAR Network Targeted Lab Hub

项目摘要 目标分析资源是一个多学科、全方位服务暴露的生物标记物计划，旨在 将无机和有机环境措施整合到现有的NIH研究中。在这份增刊中，我们现在 扩大服务范围，包括在危重期间接触对妇女和儿童健康构成风险的杀虫剂 孕期、幼儿期等窗口。这些杀虫剂不容易在尿液中检测到，因为 半衰期很短，但我们已经开发出在头发中测量它们的方法。我们每周举行一次高管指导 全体实验室中心团队的委员会会议，讨论工作、排除工作流程故障并确定资源的优先顺序 和活动，以便为HHEAR客户提供最先进的服务。我们队有相当多的 将靶向暴露生物标记物的潜在毒理学与研究设计、 收集的生物标本类型，以及对健康表型的测量，在这种情况下头发杀虫剂。我们的 实验室可行性评估有助于形成客户提交更高影响研究的科学依据。我们 还建议客户防止在被认为可能低于以下标准的检测中使用珍贵样本 检测限值是基于我们多年的研究经验和对暴露情景的理解。 我们与行政核心合作协调服务，包括样品管理、接收、 头发样本的标记和跟踪。我们作为协调中心的专家顾问， PI/客户端和其他HHEAR资源。我们与数据中心协作管理结果 报告，确保数据格式正确且具有足够的元数据，以及参与 暴露/表型关系的分析。我们使用自动化的机器人处理和制备样品 方法、高通量分析程序和实验室间最先进的QA/QC程序 包括通过最先进实验室进行实时层析监控和数据库管理 信息管理系统(LIMS)软件。我们还自动化了数据处理，以减少人为错误。 我们的方法确保了适当的样本跟踪和数据报告，并避免了样本与结果的不匹配。 在拥有大量机构资源的情况下，有针对性的资源使 自成立以来，质谱仪的数量增加到16台，确保了我们服务于需求的能力 HHEAR网络的成员。我们的团队还进行广泛的外展，是唯一的HHEAR实验室中心 与The Mountain合作，在美国、欧洲和墨西哥举办国际博览会 西奈半岛非目标实验室中心。所有目标化合物的QA/QC协议和标准操作程序， 包括作为这一应用的重点的杀虫剂，已经被建立以保持我们的 化学分析。我们开发了元素和有机化学品的小组，这些小组总共评估了超过 200种化学品。我们参与了HHEAR规定的所有金属和有机产品的QA/QC和PT计划 化学品来验证我们的精确度和准确性。

Project Summary The Targeted Analysis Resource is a multi-disciplinary, full service exposure biomarker program designed to integrate inorganic and organic environmental measures into existing NIH studies. In this supplement we now extend services to include exposure to pesticides that pose health risk to women and children during critical windows such as pregnancy and early childhood. These pesticides are not readily measured in urine due to short half-lives but we have developed methods to measure them in hair. We hold weekly Executive Steering Committee meetings of the full Lab Hub team to discuss jobs, troubleshoot workflows, and prioritize resources and activities in order to provide state of the art service to HHEAR clients. Our team has considerable expertise in matching the underlying toxicology of targeted exposure biomarkers to the study design, to the biological specimen types collected, and to the health phenotypes measured, in this case hair pesticides. Our lab feasibility assessments help shape the science of a client’s submission towards higher impact studies. We also counsel clients on preventing the use of precious samples on assays that are deemed likely to be below detection limits based on our many years of research experience and understanding of exposure scenarios. We work with the Administrative Core to coordinate services, including sample management, receiving, labeling and tracking of the hair samples. We serve as expert consultants with the Coordinating Center, PI/Clients and other HHEAR Resources. We collaborate with the Data Center in the management of results reporting, ensuring that data are properly formatted and have sufficient metadata, as well as participate in the analysis of exposure/phenotype relationships. We use automated robotic sample handling and preparation methods, high throughput analytical procedures and state of the art inter-laboratory QA/QC procedures which include real time chromatography monitoring and database management through state-of-the-art laboratory information management system (LIMS) software. We also automated data processing to reduce human error. Our methods ensure proper sample tracking and data reporting and avoid mismatches of samples to results. With substantial institutional resources at its disposal, the Targeted Resource has more than doubled the number of mass spectrometry instruments to 16 since our inception, ensuring our capacity to serve the needs of the HHEAR Network. Our team also conducts extensive outreach and is the only HHEAR lab hub to organize an international Exposome Symposia in the US, Europe and Mexico, in collaboration with the Mount Sinai Untargeted Lab Hub. QA/QC protocols and standard operating procedures for all target compounds, including the pesticides that are the focus of this application, have been established to maintain the rigor of our chemical analyses. We developed panels for elements and organic chemicals that in sum assess more than 200 chemicals. We participate in all HHEAR mandated QA/QC and PT programs for metals and organic chemicals to verify our precision and accuracy.