Regulatory Mechanisms of Meiosis in Fission Yeast

裂殖酵母减数分裂的调控机制

• 批准号: 21227007
• 负责人: YAMAMOTO Masayuki
• 金额: $ 132.95万
• 依托单位: Kazusa DNA Research Institute (2012-2013)The University of Tokyo (2009-2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (S)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009-05-11 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21227007/
• 关键词: 遺伝学; 発生・分化; 減数分裂; 情報伝達; RNA; 微小管

We investigated the regulatory mechanisms of meiosis in fission yeast. Certain mRNAs required for meiosis are transcribed but undergo degradation called "selective elimination" in vegetatively growing cells. We analyzed this mechanism and identified a number of molecules involved. A pivotal factor Mmi1 protein recognized and bound to specific hexanucleotide motifs on the target mRNAs. meiRNA, which inactivates Mmi1 to promote meiosis, carried numerous copies of the hexanucleotide motifs and lured Mmi1. Meanwhile, we found that phosphorylation of Ser-2 residues on the C-terminal domain (CTD) of RNA polymerase II was essential to transcribe a specific gene required for the initiation of meiosis, and that the stress-responsive MAP kinase pathway was involved in this regulation.

我们研究了裂殖酵母减数分裂的调控机制。减数分裂所需的某些mRNAs是转录的，但在营养生长的细胞中会经历被称为“选择性消除”的降解。我们分析了这一机制，并确定了一些相关的分子。识别并结合靶mRNAs上特定六核苷酸基序的关键因子Mmi1蛋白。MeiRNA可以灭活Mmi1以促进减数分裂，它携带大量的六核苷酸基序，并引诱Mmi1。同时，我们发现RNA聚合酶II的C末端结构域(CTD)上的Ser-2残基的磷酸化对于转录启动减数分裂所需的特定基因是必不可少的，并且胁迫响应的MAPK通路参与了这一调节。

项目成果

Nuclear Compartmentalization Is Abolished during Fission Yeast Meiosis

• DOI: 10.1016/j.cub.2010.09.004
• 发表时间: 2010-11-09
• 期刊: CURRENT BIOLOGY
• 影响因子: 9.2
• 作者: Arai, Kunio;Sato, Masamitsu;Yamamoto, Masayuki
• 通讯作者: Yamamoto, Masayuki

Control of meiosis by RNA destruction in fission yeast

通过破坏裂殖酵母中的 RNA 来控制减数分裂

• 发表时间: 2012
• 作者: 野口遼太;松島紀佐;佐々木大輔;中橋和博;M. Yamamoto
• 通讯作者: M. Yamamoto

SPB components are reorganized during fission yeast meiosis

SPB 成分在裂殖酵母减数分裂过程中重组

• 发表时间: 2012
• 期刊: Mol. Biol. Cell
• 作者: M. Ohta;M. Sato and M. Yamamoto
• 通讯作者: M. Sato and M. Yamamoto

Pob1 Ensures Cylindrical Cell Shape by Coupling Two Distinct Rho Signaling Events During Secretory Vesicle Targeting

• DOI: 10.1111/j.1600-0854.2011.01190.x
• 发表时间: 2011-06
• 期刊: Traffic
• 影响因子: 4.5
• 作者: K. Nakano;M. Toya;A. Yoneda;Y. Asami;A. Yamashita;N. Kamasawa;M. Osumi;Masayuki Yamamoto

Psk1, an AGC kinase family member in fission yeast, is directly phosphorylated and controlled by TORC1 and functions as S6 kinase

• DOI: 10.1242/jcs.111146
• 发表时间: 2012-12-01
• 期刊: JOURNAL OF CELL SCIENCE
• 影响因子: 4
• 作者: Nakashima, Akio;Otsubo, Yoko;Tamanoi, Fuyuhiko
• 通讯作者: Tamanoi, Fuyuhiko