Regulatory Mechanisms of Meiosis in Fission Yeast

研究了分裂酵母减数分裂的调控机制。在无性生长的细胞中，减数分裂所需的某些mrna被转录，但会经历被称为“选择性消除”的降解。我们分析了这一机制，并确定了一些相关分子。关键因子Mmi1蛋白识别并结合到目标mrna上的特定六核苷酸基序。meiRNA可以使Mmi1失活以促进减数分裂，携带大量的六核苷酸基序拷贝并吸引Mmi1。同时，我们发现RNA聚合酶II c端结构域（CTD）上Ser-2残基的磷酸化对于转录减数分裂起始所需的特定基因至关重要，并且应激反应性MAP激酶途径参与了这一调控。

We investigated the regulatory mechanisms of meiosis in fission yeast. Certain mRNAs required for meiosis are transcribed but undergo degradation called "selective elimination" in vegetatively growing cells. We analyzed this mechanism and identified a number of molecules involved. A pivotal factor Mmi1 protein recognized and bound to specific hexanucleotide motifs on the target mRNAs. meiRNA, which inactivates Mmi1 to promote meiosis, carried numerous copies of the hexanucleotide motifs and lured Mmi1. Meanwhile, we found that phosphorylation of Ser-2 residues on the C-terminal domain (CTD) of RNA polymerase II was essential to transcribe a specific gene required for the initiation of meiosis, and that the stress-responsive MAP kinase pathway was involved in this regulation.