Regulatory Mechanisms of Meiosis in Fission Yeast

研究了裂殖酵母减数分裂的调控机制。减数分裂所需的某些mRNA被转录，但在植物生长细胞中经历称为“选择性消除”的降解。我们分析了这一机制，并确定了一些分子参与。关键因子Mmi 1蛋白识别并结合靶mRNA上的特异性六核苷酸基序。使Mmi 1失活以促进减数分裂的meiRNA携带了许多拷贝的六核苷酸基序并引诱Mmi 1。同时，我们发现RNA聚合酶II C端结构域（CTD）上Ser-2残基的磷酸化对于转录减数分裂起始所需的特定基因是必不可少的，并且胁迫响应MAP激酶途径参与了这一调节。

We investigated the regulatory mechanisms of meiosis in fission yeast. Certain mRNAs required for meiosis are transcribed but undergo degradation called "selective elimination" in vegetatively growing cells. We analyzed this mechanism and identified a number of molecules involved. A pivotal factor Mmi1 protein recognized and bound to specific hexanucleotide motifs on the target mRNAs. meiRNA, which inactivates Mmi1 to promote meiosis, carried numerous copies of the hexanucleotide motifs and lured Mmi1. Meanwhile, we found that phosphorylation of Ser-2 residues on the C-terminal domain (CTD) of RNA polymerase II was essential to transcribe a specific gene required for the initiation of meiosis, and that the stress-responsive MAP kinase pathway was involved in this regulation.