JOEL JEM-1200 EXII ELECTRON MICROSCOPE

本申请是对申请#S10-RR08374-01的重新提交，申请资金为 购买JEOL 1200EXII透射电子显微镜(TEM)。这 仪器将作为主要的高分辨率显微镜在 新近成立的结构生物学成像中心(SBIC) 匹兹堡大学医学院。这一机构的任务是 提供全方位的电子光学、图像分析和 向医学领域内的所有研究部门提供形态测量方法。 在我们上次申请后的一年里，SBIC已经成为一种 医学研究界不可或缺的一部分，参与研究 有40多个小灵通资助的研究小组参与的项目 区域。目前的瞬变电磁设备基地由JEOL 100CX和JEOL组成 100亿美元。 SBIC开幕时，指导委员会与 该中心和不同的用户群体明确表示， 现有的电子显微镜在这两项技术上都是不够的。 以及服务于研究界需求的能力。我们的 在第一次拨款申请时的预测， 在设施内使用显微镜的时间将达到溢价 当SBIC完全发挥作用时，确实已经在 过去16个月。可用仪器时间进一步受到以下因素的影响 JEOL 100B一贯的不可靠性。这台老式显微镜有 功能有限，仅可由符合以下条件的专家用户操作 了解这件乐器的古怪之处。它不能被学生或 由经验较少的用户提供。由于这些问题，我们现在 仅限于使用我们剩余的显微镜，它不能也不能 提供足够的显微镜工作时间以满足用户需求。 在我们之前的申请中，我们要求为配备了1200EXII的 带有测角台和自动蒙太奇装置。这些功能包括 被认为是不合理的，因此从本文件中省略 申请。购买它们的资金得到了 制度保障。因此，我们将当前应用程序的重点放在 用于购买现有最便宜的120千伏仪器的资金 在今天的市场上。把这个显微镜加到SBIC上将会 使我们能够从密集的日常服务中淘汰100B，并提供 高质量的仪器和充足的显微镜时间让我们永远- 不断扩大的用户群。

This request is a resubmission of application #S10-RR08374-01 for funds to purchase a JEOL 1200EXII transmission electron microscope (TEM). This instrument will serve as the primary high resolution microscope within the recently established Structural Biology Imaging Center (SBIC) of the University of Pittsburgh Medical School. The mandate of this facility is to provide access to a full range of electron optical, image analysis, and morphometric methods to all research departments within the medical area. In the year since our previous application, the SBIC has become an integral part of the medical research community, participating in research projects with more than 40 PHS funded research groups within the medical area. The current TEM equipment base consists of a JEOL 100CX and a JEOL 100B. When the SBIC was opened, consultations between the steering committee of the center and the different user groups clearly indicated that the existing electron microscopes were inadequate in terms of both technology and the ability to serve the needs of the research community. Our prediction, at the time of the first grant application, that the availability of microscope time within the facility would reach a premium when the SBIC became fully functional, has indeed been borne out in the past 16 months. Available instrument time has been further compromised by the consistent unreliability of the JEOL 100B. This older microscope has limited functionality and is operable only by "expert" users who understand the quirks of this instrument. It cannot be used by students or by less experienced users. As a result of these problems, we are now limited to the use of our remaining microscope, which cannot and does not provide enough microscope hours to satisfy user demands. In our previous application, we requested funding for a 1200EXII equipped with a goniometer stage and an automontage device. These features were deemed unjustified and, accordingly, have been omitted from the current application. Funds for their purchase have been guaranteed by institutional support. Thus, we have focused our current application to funding for the purchase of the least expensive l20kV instrument available on the market today. The addition of this microscope to the SBIC would enable us to retire the 100B from intensive daily service and to provide high quality instrumentation and sufficient microscope time to our ever- expanding group of users.