JOEL JEM-1200 EXII ELECTRON MICROSCOPE

本申请是重新提交申请#S10-RR 08374 -01，以获得资金， 购买JEOL 1200 EXII透射电子显微镜（TEM）。这 仪器将作为主要的高分辨率显微镜内， 结构生物学成像中心（SBIC） 匹兹堡大学医学院。该设施的任务是 提供全方位的电子光学，图像分析， 医学领域内的所有研究部门的形态测量方法。 自我们上次申请以来的一年中，SBIC已成为 医学研究界的组成部分，参与研究 项目与40多个小灵通资助的医疗内研究小组 区目前的TEM设备基础由JEOL 100CX和JEOL 100CX组成。 100 B. 当SBIC开幕时， 该中心和不同的用户群体清楚地表明， 现有的电子显微镜在这两种技术方面都是不够的， 以及满足研究团体需求的能力。我们 在第一次申请补助金时， 在设施内的显微镜时间的可用性将达到溢价 当SBIC完全发挥作用时， 过去16个月可用仪器时间进一步受到以下因素的影响： JEOL 100 B的一贯不可靠性。这台老式的显微镜 功能有限，只能由“专家”用户操作， 了解这台仪器的古怪之处学生不能使用， 经验较少的用户。由于这些问题，我们现在 仅限于使用我们剩下的显微镜，它不能也不会 提供足够的显微镜小时数以满足用户需求。 在我们之前的申请中，我们申请了1200 EXII的资金， 带有测角台和自动拼接装置 这些特征 被认为是不合理的，因此，已从目前的省略 应用程序.购买的资金由 机构支持。 因此，我们将当前的应用集中于 为购买最便宜的120千伏仪器提供资金 在今天的市场上。将这种显微镜添加到SBIC将 使我们能够从密集的日常服务中淘汰100 B，并提供 高质量的仪器和充足的显微镜时间，我们永远- 扩大用户群。

This request is a resubmission of application #S10-RR08374-01 for funds to purchase a JEOL 1200EXII transmission electron microscope (TEM). This instrument will serve as the primary high resolution microscope within the recently established Structural Biology Imaging Center (SBIC) of the University of Pittsburgh Medical School. The mandate of this facility is to provide access to a full range of electron optical, image analysis, and morphometric methods to all research departments within the medical area. In the year since our previous application, the SBIC has become an integral part of the medical research community, participating in research projects with more than 40 PHS funded research groups within the medical area. The current TEM equipment base consists of a JEOL 100CX and a JEOL 100B. When the SBIC was opened, consultations between the steering committee of the center and the different user groups clearly indicated that the existing electron microscopes were inadequate in terms of both technology and the ability to serve the needs of the research community. Our prediction, at the time of the first grant application, that the availability of microscope time within the facility would reach a premium when the SBIC became fully functional, has indeed been borne out in the past 16 months. Available instrument time has been further compromised by the consistent unreliability of the JEOL 100B. This older microscope has limited functionality and is operable only by "expert" users who understand the quirks of this instrument. It cannot be used by students or by less experienced users. As a result of these problems, we are now limited to the use of our remaining microscope, which cannot and does not provide enough microscope hours to satisfy user demands. In our previous application, we requested funding for a 1200EXII equipped with a goniometer stage and an automontage device. These features were deemed unjustified and, accordingly, have been omitted from the current application. Funds for their purchase have been guaranteed by institutional support. Thus, we have focused our current application to funding for the purchase of the least expensive l20kV instrument available on the market today. The addition of this microscope to the SBIC would enable us to retire the 100B from intensive daily service and to provide high quality instrumentation and sufficient microscope time to our ever- expanding group of users.