TISSUE COMPOSITION W/ ATTENUATED TOTAL REFLECTION INFRARED MICROSPECTROSCOPY

采用衰减全反射红外显微镜的组织成分

• 批准号: 6205738
• 负责人: LISA M MILLER
• 金额: --
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6205738
• 关键词: biological products; biomedical resource; proteins; spectrometry; technology /technique

Using a small volume mixer with the "continuous flow" method, the flow speed can be made very high, which accelerates the generation of turbulence in the sample flow and thus promotes efficient mixing. Dr. Denis Rousseau has developed such a mixer for resonance Raman and fluorescence spectroscopies, and we have modified the design for use with the synchrotron infrared beam. The mixer is designed such that two solutions are loaded into syringes and pumped at 2 mm/ms into a small mixing well (100 ?m wide x 100 ?m deep) etched into a stainless steel block. The mixing well is covered by a 50 ?m-thick teflon sheet, which has a 200 ?m-diameter hole aligned in the center of the mixing well. The solutions mix in the mixing well and flow through the teflon spacer into the infrared "sandwich" cell. The cell is made of two, rectangular CaF2 windows (12 mm x 25 mm), separated by a mylar Hspacer (100 ?m thick). The spacer creates a channel 200 ?m wide Hthrough the cell and provides a suitable infrared pathlength. By Hmoving the position of the infrared beam along the length of the infrared cell, different time points in the folding reaction can be probed. Since the dead time of the mixer is ~100 ?s, the time resolution of this rapid-mix flow cell is on the order of hundreds of microseconds to a few milliseconds.

Using a small volume mixer with the "continuous flow" method, the flow speed can be made very high, which accelerates the generation of turbulence in the sample flow and thus promotes efficient mixing. 博士。 Denis Rousseau has developed such a mixer for resonance Raman and fluorescence spectroscopies, and we have modified the design for use 与同步加速器红外光束。 混合器的设计使得 two solutions are loaded into syringes and pumped at 2 mm/ms into a small mixing well (100 ?m wide x 100 ?m deep) etched into a stainless 钢块。 The mixing well is covered by a 50 ?m-thick teflon 片材，其中心有一个直径为 200 微米的孔 搅拌均匀。 溶液在混合井中混合并流过 the teflon spacer into the infrared "sandwich" cell. 细胞已制成 两个矩形 CaF2 窗口 (12 mm x 25 mm)，由聚酯薄膜隔开 H垫片（100微米厚）。 垫片形成一个 200 微米宽的通道 H穿过电池并提供合适的红外光程。 经过 沿着物体的长度移动红外光束的位置 红外池，折叠反应的不同时间点可以 探查。 由于混频器的死区时间约为 100 µs，因此时间 这种快速混合流动池的分辨率约为数百 微秒到几毫秒。