TISSUE COMPOSITION W/ ATTENUATED TOTAL REFLECTION INFRARED MICROSPECTROSCOPY

使用具有“连续流动”方法的小体积混合器， 可以使流速非常高，这加速了 样品流中的湍流，从而促进有效的混合。 博士 Denis Rousseau已经开发了这样一种用于共振拉曼的混频器， 荧光光谱，我们已经修改了设计， 同步加速器红外光束 混频器设计成 将两种溶液装入注射器中并以2 mm/ms泵入 小混合孔（100？m宽x 100？米深）蚀刻成不锈钢 钢块 混合井是由一个50？m厚碲化镉 #21040;，其中有200？m直径的孔对准的中心 混合均匀。 溶液在混合孔中混合并流过 将碲化镉间隔物放入红外“三明治”单元中。 细胞是由 两个矩形CaF 2窗口（12 mm x 25 mm），由聚酯薄膜隔开 Hspacer（100？m厚）。 间隔件产生通道200？米宽 并提供合适的红外路径长度。 通过 H沿着红外线光束的长度沿着移动红外线光束的位置， 红外细胞，在折叠反应的不同时间点， 试探 由于混频器的死区时间约为100？s，时间 这种快速混合流动池的分辨率大约为数百微米 微秒到几毫秒。

Using a small volume mixer with the "continuous flow" method, the flow speed can be made very high, which accelerates the generation of turbulence in the sample flow and thus promotes efficient mixing. Dr. Denis Rousseau has developed such a mixer for resonance Raman and fluorescence spectroscopies, and we have modified the design for use with the synchrotron infrared beam. The mixer is designed such that two solutions are loaded into syringes and pumped at 2 mm/ms into a small mixing well (100 ?m wide x 100 ?m deep) etched into a stainless steel block. The mixing well is covered by a 50 ?m-thick teflon sheet, which has a 200 ?m-diameter hole aligned in the center of the mixing well. The solutions mix in the mixing well and flow through the teflon spacer into the infrared "sandwich" cell. The cell is made of two, rectangular CaF2 windows (12 mm x 25 mm), separated by a mylar Hspacer (100 ?m thick). The spacer creates a channel 200 ?m wide Hthrough the cell and provides a suitable infrared pathlength. By Hmoving the position of the infrared beam along the length of the infrared cell, different time points in the folding reaction can be probed. Since the dead time of the mixer is ~100 ?s, the time resolution of this rapid-mix flow cell is on the order of hundreds of microseconds to a few milliseconds.