Analyses on a molecular mechanism of spontaneous articular cartilage regeneration induced in vivo by double-network gel

(1)利用微阵列技术明确了双网凝胶（DN）自发再生关节软骨的遗传特征。(2)我们发现DN凝胶成分PAMPS具有诱导关节软骨自发再生的能力。(3)我们使用多色生物发光探针观察了软骨分化过程中的标记基因。我们发现了在软骨形成前凝结过程中细胞代谢的同步振荡现象。(4)研制了用于体内成像的转基因生物发光小鼠。(5)这些结果为阐明软骨自发再生现象的综合机制提供了重要信息。

(1) We clarified genetic characteristics of the articular cartilage spontaneously regenerated by double-network (DN) gel, using microarray. (2) We found that PAMPS, a component of the DN gel, has an ability that can induces the spontaneous articular cartilage regeneration. (3) We observed marker genes in a chondrogenic differentiation process, using multi-colored bioluminescence probes. We discovered a synchronized oscillation phenomenon of cell metabolism in prechondrogenic condensation. (4) We developed the transgenic bioluminescence mouse to be used for in vivo imaging. (5) These results provided important information to clarify a comprehensive mechanism of the spontaneous cartilage regeneration phenomenon.