Application of DNA microarray technique to mutagenesis assessment.

将高浓度或低浓度丝裂霉素C(MMC)分别作用于4.0微克/毫升植物血凝素(PHA)刺激的淋巴母细胞。染毒24小时后回收总RNA，用DNA芯片分析基因表达谱。胸腺肽α原有明显变化。用2.5微克/毫升的PHA也做了同样的实验，因为没有发现与细胞周期停滞或DNA修复有关的探针。然而，只有共比例卟啉原氧化酶和28S rRNA发生了显著变化。MMC会导致双链DNA的链间交联，但它的失真据说很小。这可能是导致阴性结果的原因。将高浓度或低浓度的N-甲基-亚硝脲(MNU)暴露于2.5微克/毫升的PHA刺激的淋巴母细胞中。染毒24小时后回收总RNA，用DNA芯片分析基因表达谱。MNU以鸟嘌呤残基的O6甲基化而闻名，氧化应激由MNU诱导。与细胞周期停滞、DNA修复或氧化应激反应相关的探针显示显著变化。重新分析BLM、UVA或UVC数据显示相应的细胞对损伤的反应。

High or low concentration mitomycin C (MMC) was exposed to lymphoblast stimulated by 4.0 micro g/ml of phytohemagglutinin (PHA). Total RNA was recovered 24 after exposure and expression profile was analyzed with DNA microarray. Prothymosin alpha showed significant change. Same experiment was done with 2.5 micro g/ml of PHA, because no probe relating cell cycle arrest or DNA repair was identified. However, significant change was observed only for coproporphyrinogen oxidase and 28S rRNA. MMC causes inter strand cross-link for double strand DNA but its distortion is said to be small. This might be the reason for the negative result.High or low concentration n-methyl-nitrosourea (MNU) was exposed to lymphoblast stimulated by 2.5 micro g/ml of PHA. Total RNA was recovered 24 after exposure and expression profile was analyzed with DNA microarray. MNU is famous with O6 methylation of guanine residue and oxidative stress is induced by MNU. Probes related with cell cycle arrest, DNA repair or oxidative stress response showed significant change.Reanalysis of BLM, UVA or UVC data revealed corresponding cellular response to the lesion.